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Titolo:
Construction of a 1.2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirustrifoliata (L.) Raf.
Autore:
Yang, ZN; Ye, XR; Choi, SD; Molina, J; Moonan, F; Wing, RA; Roose, ML; Mirkov, TE;
Indirizzi:
Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 Sci, Riverside, CA 92521 USA Texas A&M Univ, Agr Expt Stn, Dept Plant Pathol & Microbiol, Weslaco, TX 78596 USA Texas A&M Univ Weslaco TX USA 78596 ol & Microbiol, Weslaco, TX 78596 USA Clemson Univ, Genome Ctr, Clemson, SC 29634 USA Clemson Univ Clemson SC USA 29634 Univ, Genome Ctr, Clemson, SC 29634 USA
Titolo Testata:
GENOME
fascicolo: 3, volume: 44, anno: 2001,
pagine: 382 - 393
SICI:
0831-2796(200106)44:3<382:COA1CI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-BINDING SITE; TOBACCO ETCH VIRUS; LINKAGE MAP; BAC LIBRARY; ARABIDOPSIS; DNA; REGION; RESTRICTION; PROTEIN; PROGRAMS;
Keywords:
Poncirus; citrus tristeza virus; chromosome walk; resistance gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Mirkov, TE Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521USA Univ Calif Riverside Riverside CA USA 92521 side, CA 92521 USA
Citazione:
Z.N. Yang et al., "Construction of a 1.2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirustrifoliata (L.) Raf.", GENOME, 44(3), 2001, pp. 382-393

Abstract

The citrus tristeza virus resistance gene (Ctv) is a single dominant gene in Poncirus trifoliata, a sexually compatible relative of citrus. To clone this gene, a bacterial artificial chromosome (BAC) library has been constructed from an individual plant that was homozygous for Ctv. This library contains 45 696 clones with an average insert size of 80 kb, corresponding to 9.6 genome equivalents. Screening of the BAC library with five chloroplast DNA probes indicated that 0.58% of the BAC clones contained chloroplast-derived inserts. The chromosome walk across the Ctv locus was initiated using three closely linked genetic markers: C19, AD8, and Z16. The walk has been completed and a contig of ca. 1.2 Mb was constructed. Based on new data, the genetic map in the Ctv region was revised, with Ctv being located betweenAD8-Z16 and C19 at distances of 1.2 and 0.6 cM, respectively. Utilizing DNA fragments isolated from the contig as RFLP markers, the Ctv locus was further mapped to a region of ca. 300 kb. This contig contains several putative disease-resistance genes similar to the rice Xa21 gene, the tomato Cf-2 gene, and the Arabidopsis thaliana RPS2 gene. This library will therefore allow cloning of Ctv and other putative disease-resistance genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:36:41