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Titolo:
Age-related mitochondrial genotypic and phenotypic alterations in human skeletal muscle
Autore:
Pesce, V; Cormio, A; Fracasso, F; Vecchiet, J; Felzani, G; Lezza, AMS; Cantatore, P; Gadaleta, MN;
Indirizzi:
Univ Bari, Dept Biochem & Mol Biol, I-70125 Bari, Italy Univ Bari Bari Italy I-70125 ept Biochem & Mol Biol, I-70125 Bari, Italy Ctr Study Mitochondria & Energet Metab, Bari, Italy Ctr Study Mitochondria& Energet Metab Bari Italy et Metab, Bari, Italy Univ Chieti, Dept Internal Med & Aging, Chieti, Italy Univ Chieti ChietiItaly ieti, Dept Internal Med & Aging, Chieti, Italy
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 11, volume: 30, anno: 2001,
pagine: 1223 - 1233
SICI:
0891-5849(20010601)30:11<1223:AMGAPA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHROME-C-OXIDASE; ACETYL-L-CARNITINE; ADENINE-NUCLEOTIDE TRANSLOCASE; RAT-HEART MITOCHONDRIA; LARGE-SCALE DELETIONS; DNA DELETIONS; OXIDATIVE STRESS; POINT MUTATIONS; CONTROL REGION; SENESCENT RAT;
Keywords:
aging; mitochondria; histochemistry; MtDNA deletions; MtDNA content; free radicals; human skeletal muscle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Gadaleta, MN Univ Bari, Dept Biochem & Mol Biol, Via Orabona 4, I-70125 Bari, Italy Univ Bari Via Orabona 4 Bari Italy I-70125 70125 Bari, Italy
Citazione:
V. Pesce et al., "Age-related mitochondrial genotypic and phenotypic alterations in human skeletal muscle", FREE RAD B, 30(11), 2001, pp. 1223-1233

Abstract

To have a clearer picture of how mitochondrial damages are associated to aging, a comprehensive study of phenotypic and genotypic alterations was carried out, analyzing with histochemical and molecular biology techniques thesame skeletal muscle specimens of a large number of healthy subjects from 13 to 92 years old. Histochemical data showed that ragged red fibers (RRF) appear at about 40 years of age and are mostly cytochrome c oxidase (COX)-positive, whereas they are almost all COX-negative thereafter. Molecular analyses showed that the 4977 bp deletion of mitochondrial DNA (mtDNA(4977)) and the 7436 bp deletion of mtDNA (mtDNA(7436)), already present in individuals younger than 40 years of age, but their occurrence does not change withage. After 40 years of age the number of mtDNA deleted species, as revealed by Long Extension PCR (LX-PCR), increases, the 10422 bp deletion of mtDNA(mtDNA(10422)) appears, although with a very low frequency of occurrence, and mtDNA content is more than doubled. Furthermore, mtDNA(4977) level directly correlates with that of COX-negative fibers in the same analyzed subjects. These data clearly show that, after 40 years of age, the phenotypic and genotypic mitochondrial alterations here studied appear in human skeletalmuscle and that they are closely related. (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:20:49