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Titolo:
The effects of SCF/G-CSF prestimulation on radiation sensitivity and engraftment in nonmyeloablated murine hosts
Autore:
Giri, N; Kaushiva, A; Wu, T; Sellers, SE; Tisdale, JF;
Indirizzi:
NIDDK, Mol & Clin Hematol Branch, NIH, Bethesda, MD 20892 USA NIDDK Bethesda MD USA 20892 n Hematol Branch, NIH, Bethesda, MD 20892 USA NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 , Hematol Branch, NIH, Bethesda, MD 20892 USA
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 6, volume: 29, anno: 2001,
pagine: 779 - 785
SICI:
0301-472X(200106)29:6<779:TEOSPO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; STEM-CELL FACTOR; CHRONIC GRANULOMATOUS-DISEASE; RHESUS PERIPHERAL-BLOOD; LONG-TERM ENGRAFTMENT; BONE-MARROW; REPOPULATING CELLS; GENE-TRANSFER; HEMATOPOIETIC PROGENITOR; NONHUMAN-PRIMATES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Tisdale, JF NIDDK, Mol & Clin Hematol Branch, NIH, Bldg 10,Room 9N116,9000Rockville Pike, Bethesda, MD 20892 USA NIDDK Bldg 10,Room 9N116,9000 Rockville Pike Bethesda MD USA 20892
Citazione:
N. Giri et al., "The effects of SCF/G-CSF prestimulation on radiation sensitivity and engraftment in nonmyeloablated murine hosts", EXP HEMATOL, 29(6), 2001, pp. 779-785

Abstract

Objective. Previous studies have shown improved engraftment in a murine model when granulocyte colony-stimulating factor (G-CSF) and stem cell factor(SCF) were administered for 5 days prior to irradiation, with significant levels of engraftment in the growth factor-preconditioned group even at very low radiation doses. We sought to explore the mechanisms behind this effect,Methods. The radiation sensitivity of mice with or without 5 days of prestimulation with G-CSF (200 mug/kg/d) and SCF (50 mug/kg/d) was compared. To further evaluate whether growth factor prestimulation enhances engraftment by mobilization of hematopoietic progenitors into peripheral blood, thus creating less endogenous competition within the marrow compartment, female mice were pretreated with 5 days of G-CSF/SCF or control diluent, Engraftmentof 40 X 10(6) peripheral blood stem cells (PBSCs) harvested from G-CSF/SCF-mobilized male mice was compared in the two recipient groups. Results, There was no difference in survival between the pretreated and control mice at the radiation doses tested. Additionally, there was no significant difference in the recovery of blood counts, bone marrow cellularity, colony-forming unit (CFU) content, or stem cell numbers assessed 4 months later in a competitive repopulation model. Engraftment levels of male cells did not differ between G-CSF/SCF-pretreated and control recipients, and could be detected in 30% of recipients at 20-24 weeks (4/12 in each group) at overall levels of 0.1-1%,Conclusions. The enhanced engraftment in cytokine pretreated recipients isunlikely to be due to increased endogenous stem-cell killing or to the creation of endogenous marrow "space" by egress of endogenous stem cells aftercytokine prestimulation. (C) 2001 International Society for Experimental Hematology. Published by Elsevier Science Inc.

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Documento generato il 20/01/20 alle ore 22:56:01