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Titolo:
Isolation and characterization of canine hematopoietic progenitor cells
Autore:
Niemeyer, GP; Hudson, J; Bridgman, R; Spano, J; Nash, RA; Lothrop, CD;
Indirizzi:
Auburn Univ, Coll Vet Med, Scott Ritchey Res Ctr, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 ott Ritchey Res Ctr, Auburn, AL 36849 USA Auburn Univ, Coll Vet Med, Dept Small Anim Surg & Med, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 all Anim Surg & Med, Auburn, AL 36849 USA Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 Med, Dept Pathobiol, Auburn, AL 36849 USA Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA Fred Hutchinson Canc Res Ctr Seattle WA USA 98104 , Seattle, WA 98104 USA
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 6, volume: 29, anno: 2001,
pagine: 686 - 693
SICI:
0301-472X(200106)29:6<686:IACOCH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BONE-MARROW; C-KIT LIGAND; STEM-CELLS; PERIPHERAL-BLOOD; NOD/SCID MICE; MULTILINEAGE HEMATOPOIESIS; MONOCLONAL-ANTIBODIES; FLT3 LIGAND; CORD-BLOOD; SCID MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Lothrop, CD Auburn Univ, Coll Vet Med, Scott Ritchey Res Ctr, Auburn, AL 36849 USA Auburn Univ Auburn AL USA 36849 Res Ctr, Auburn, AL 36849 USA
Citazione:
G.P. Niemeyer et al., "Isolation and characterization of canine hematopoietic progenitor cells", EXP HEMATOL, 29(6), 2001, pp. 686-693

Abstract

Objective, The purpose of this study was to purify and characterize caninehematopoietic progenitor cells for surface antigen phenotype and reconstitution ability,Methods. Canine hematopoietic progenitor cells were isolated by density gradient sedimentation, lineage depletion with monoclonal antibodies, and fluorescence-activated cell sorting (FACS) for selection of cells with low-forward and right-angle scatter that were rhodamine 123 (Rh-123)(dull). Isolated cells were characterized for expression of CD34, c-kit, and Flt-3, A canine/murine xenograft model and a mixed-chimerism assay were used to examinethe in vivo proliferative potential of isolated cells. Results. The lineage-positive (Lin(+)) cells represented 80 +/- 11% (n = 22) of the input mononuclear cells, Lineage depletion resulted in a fourfoldincrease in colony-forming unit granulocyte/monocyte (CFU-GM), a 2.5-fold increase in burst-forming unit-erythroid (BFU-E), and a twofold increase inthe number of Rh-123(dull) cells over nonlineage-depleted bone marrow mononuclear cells (BMMCs). Lineage depletion led to a 2.7-fold enrichment of CD34 cells, a 10.4-fold enrichment of c-kit cells, and a 10.8-fold enrichmentof CD34/c-kit(++) cells over total BMMCs, Nineteen percent of lineage-negative (Lin) cells were positive for Flt-3, Injection of canine cells into irradiated (400 rads) NOD/SCID mice resulted in the detection of canine CD45() cells with BMMCs, Lin- cells, or Rh-123(dull) cells, Transplantation of purified Lin- cells in dog leukocyte antigen-matched littermates resulted in low-level engraftment for at least In weeks. Conclusion. The development of methods for purification and characterization of canine hematopoietic progenitor cells should enhance the utilization of the canine model for a variety of experimental and therapeutic purposes. (C) 2001 International Society for Experimental Hematology Published by Elsevier Science Inc.

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Documento generato il 18/09/20 alle ore 08:32:19