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Titolo:
Human acrocentric chromosomes with transcriptionally silent nucleolar organizer regions associate with nucleoli
Autore:
Sullivan, GJ; Bridger, JM; Cuthbert, AP; Newbold, RF; Bickmore, WA; McStay, B;
Indirizzi:
Univ Dundee, Ninewells Hosp, Biomed Res Ctr, Dundee DD1 9SY, Scotland UnivDundee Dundee Scotland DD1 9SY ed Res Ctr, Dundee DD1 9SY, Scotland Brunel Univ, Dept Sci Biol, Uxbridge UB8 3PH, Middx, England Brunel Univ Uxbridge Middx England UB8 3PH bridge UB8 3PH, Middx, England MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland MRC Edinburgh Midlothian Scotland EH4 2XU h EH4 2XU, Midlothian, Scotland
Titolo Testata:
EMBO JOURNAL
fascicolo: 11, volume: 20, anno: 2001,
pagine: 2867 - 2877
SICI:
0261-4189(20010601)20:11<2867:HACWTS>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-I; RIBOSOMAL GENE-TRANSCRIPTION; TATA-BINDING PROTEIN; SPECIES-SPECIFICITY; PRENUCLEOLAR BODIES; SATELLITE DNA; FACTOR HUBF; CELLS; MITOSIS; GENOME;
Keywords:
human acrocentric chromosomes; nucleolar organizer regions; nucleoli; somatic cell hybrids; UBF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: McStay, B Univ Dundee, Ninewells Hosp, Biomed Res Ctr, Dundee DD1 9SY, Scotland Univ Dundee Dundee Scotland DD1 9SY , Dundee DD1 9SY, Scotland
Citazione:
G.J. Sullivan et al., "Human acrocentric chromosomes with transcriptionally silent nucleolar organizer regions associate with nucleoli", EMBO J, 20(11), 2001, pp. 2867-2877

Abstract

Human ribosomal gene repeats are distributed among five nucleolar organizer regions (NORs) on the p arms of acrocentric chromosomes. On exit from mitosis, nucleoli form around individual active NORs, As cells progress through the cycle, these mini-nucleoli fuse to form large nucleoli incorporating multiple NORs, It is generally assumed that nucleolar incorporation of individual NORs is dependent on ribosomal gene transcription. To test this assumption, we determined the nuclear location of individual human acrocentric chromosomes, and their associated NORs, in mouse > human cell hybrids, Human ribosomal genes are transcriptionally silent in this context. Combined immuno-fluorescence and in situ hybridization (immuno-FISH) on three-dimensional preserved nuclei showed that human acrocentric chromosomes associate with hybrid cell nucleoli. Analysis of purified nucleoli demonstrated that human and mouse NORs are equally likely to be within a hybrid cell nucleolus. This is supported further by the observation that murine upstream binding factor can associate with human NORs, Incorporation of silent NORs into mature nucleoli raises interesting issues concerning the maintenance of the activity status of individual NORs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:24:03