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Titolo:
INCENP is required for proper targeting of Survivin to the centromeres andthe anaphase spindle during mitosis
Autore:
Wheatley, SP; Carvalho, A; Vagnarelli, P; Earnshaw, WC;
Indirizzi:
Univ Edinburgh, Inst Cell & Mol Biol, Chromosome Struct Grp, Edinburgh EH93JR, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH93JR Midlothian, Scotland Univ Oporto, Inst Patol & Imunol Mol, P-4200 Oporto, Portugal Univ OportoOporto Portugal P-4200 & Imunol Mol, P-4200 Oporto, Portugal
Titolo Testata:
CURRENT BIOLOGY
fascicolo: 11, volume: 11, anno: 2001,
pagine: 886 - 890
SICI:
0960-9822(20010605)11:11<886:IIRFPT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
INNER CENTROMERE; PROTEIN INCENP; CLEAVAGE FURROW; MITOTIC SPINDLE; CELL-DIVISION; KINASE AIR-2; CYTOKINESIS; APOPTOSIS; CHROMOSOMES; MIDBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Earnshaw, WC Univ Edinburgh, Inst Cell & Mol Biol, Chromosome Struct Grp, Kings Bldgs,Mayfield Rd,Swann Bldg, Edinburgh EH9 3JR, Midlothian, ScotlandUniv Edinburgh Kings Bldgs,Mayfield Rd,Swann Bldg Edinburgh Midlothian Scotland EH9 3JR
Citazione:
S.P. Wheatley et al., "INCENP is required for proper targeting of Survivin to the centromeres andthe anaphase spindle during mitosis", CURR BIOL, 11(11), 2001, pp. 886-890

Abstract

Three lines of investigation have suggested that interactions between Survivin and the chromosomal passenger proteins INCENP and Aurora-B kinase may be important for mitotic progression, first, interference with the functionof Survivin/BIR1, INCENP, or Aurora-B kinase leads to similar defects in mitosis and cytokinesis [1-7] (see [8] for review). Second, INCENP and Aurora-B exist in a complex in Xenopus eggs [9] and in mammalian cultured cells [7], Third, interference with Survivin or INCENP function causes Aurora-B kinase to be mislocalized in mitosis in both C. elegans and vertebrates [5, 7, 9], Here, we provide evidence that Survivin, Aurora-B, and INCENP interact physically and functionally, Direct visualization of Survivin-GFP in mitotic cells reveals that it localizes identically to INCENP and Aurora-B, Survivin binds directly to both Aurora-B and INCENP in yeast two-hybrid and in vitro pull-down assays. The in vitro interaction between Survivin and Aurora-B is extraordinarily stable in that it resists 3 M NaCl. Finally, Survivin and INCENP interact functionally in vivo; in cells in which INCENP localization is disrupted, Survivin adheres to the chromosomes and no longer concentrates at the centromeres or transfers to the anaphase spindle midzone,Our data provide the first biochemical evidence that Survivin can interactdirectly with members of the chromosomal passenger complex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:37:17