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Titolo:
Gene tagging with random amplified polymorphic DNA (RAPD) markers for molecular breeding in plants
Autore:
Ranade, SA; Farooqui, N; Bhattacharya, E; Verma, A;
Indirizzi:
Natl Bot Res Inst, Plant Mol Biol Div, Lucknow 226001, Uttar Pradesh, India Natl Bot Res Inst Lucknow Uttar Pradesh India 226001 Uttar Pradesh, India
Titolo Testata:
CRITICAL REVIEWS IN PLANT SCIENCES
fascicolo: 3, volume: 20, anno: 2001,
pagine: 251 - 275
SICI:
0735-2689(2001)20:3<251:GTWRAP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BULKED SEGREGANT ANALYSIS; LEAF RUST RESISTANCE; NEAR-ISOGENIC LINES; FRAGMENT-LENGTH-POLYMORPHISMS; QUANTITATIVE TRAIT LOCI; BRASSICA-NAPUS L.; TRITICUM-AESTIVUM L; HALF-SIB FAMILY; RFLP MARKERS; AFLP MARKERS;
Keywords:
RAPD; gene tagging;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
141
Recensione:
Indirizzi per estratti:
Indirizzo: Ranade, SA Natl Bot Res Inst, Plant Mol Biol Div, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, India Natl Bot Res Inst Rana Pratap Marg Lucknow Uttar Pradesh India 226001
Citazione:
S.A. Ranade et al., "Gene tagging with random amplified polymorphic DNA (RAPD) markers for molecular breeding in plants", CR R PLANT, 20(3), 2001, pp. 251-275

Abstract

Markers are of interest to plant breeders as a source of genetic information on crops and for use in indirect selection of traits to which the markers are linked. In the classic breeding approach, the markers were invariablythe visible morphological and other phenotypic characters, and the breeders expended considerable effort and time in refining the crosses as the tight linkage or association of the desired characters with the obvious phenotypic characters was never unequivocally established. Furthermore, indirect selection for a trait using such morphological markers was not practical dueto (1) a paucity of suitable markers, (2) the undesirable pleiotropic effects of many morphological markers on plant phenotype, and (3) the inabilityto score multiple morphological mutant traits in a single segregating population. With the advancement in molecular biology, the use of molecular markers in plant breeding has become very commonplace and has given rise to "molecular breeding". Molecular breeding involves primarily "gene tagging", followed by "marker-assisted selection" of desired genes or genomes. Gene tagging refers to the identification of existing DNA or the introduction of new DNA that can function as a tag or label for the gene of interest. In order for the DNA sequences to be conserved as a tag, important prerequisites exist. This review also summarizes the achievements in gene tagging that have been made over the last 7 to 8 years.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:41:07