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Titolo:
Urocortin in the synovial tissue of patients with rheumatoid arthritis
Autore:
Uzuki, M; Sasano, H; Muramatsu, Y; Totsune, K; Takahashi, K; Oki, Y; Iino, K; Sawai, T;
Indirizzi:
Iwate Med Univ, Dept Pathol 1, Sch Med, Morioka, Iwate 0208505, Japan Iwate Med Univ Morioka Iwate Japan 0208505 Morioka, Iwate 0208505, Japan Hamamatsu Med Univ, Hamamatsu, Shizuoka 534, Japan Hamamatsu Med Univ Hamamatsu Shizuoka Japan 534 atsu, Shizuoka 534, Japan Tohoku Univ, Sch Med, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 ed, Sendai, Miyagi 9808575, Japan
Titolo Testata:
CLINICAL SCIENCE
fascicolo: 6, volume: 100, anno: 2001,
pagine: 577 - 589
SICI:
0143-5221(200106)100:6<577:UITSTO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICOTROPIN-RELEASING-FACTOR; MAST-CELL DEGRANULATION; FACTOR-RECEPTOR; VASCULAR-PERMEABILITY; BINDING-PROTEIN; MESSENGER-RNA; CRF RECEPTORS; HORMONE; EXPRESSION; IMMUNOREACTIVITY;
Keywords:
corticotropin-releasing factor; corticotropin-releasing factor receptor; rheumatoid arthritis; synovial fluid; synovium; urocortin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Uzuki, M Iwate Med Univ, Dept Pathol 1, Sch Med, 19-1 Uchimaru, Morioka, Iwate 0208505, Japan Iwate Med Univ 19-1 Uchimaru Morioka Iwate Japan 0208505 5, Japan
Citazione:
M. Uzuki et al., "Urocortin in the synovial tissue of patients with rheumatoid arthritis", CLIN SCI, 100(6), 2001, pp. 577-589

Abstract

Urocortin is a newly identified member of the corticotropin-releasing factor (CRF) neuropeptide family, and is known to be involved in the modulationof the inflammatory process. We examined the expression of urocortin, CRF and their receptors (CRF receptor; CRF-R) in the synovial tissue of patients with rheumatoid arthritis (RA) in order to study the possible biological roles of urocortin. Synovial tissues/fluids were obtained from 38 patients with RA, nine patients with osteoarthritis and four with trauma. We studiedthe concentration of urocortin in the synovial fluid using RIA, and the expression of urocortin in synovial tissue using immunohistochemistry, mRNA in situ hybridization and reverse transcriptase-PCR (RT-PCR). In addition, we examined the immunolocalization of CRF and the expression of CRF-R I, -R2-alpha and -R2-beta mRNAs utilizing RT-PCR in these synovial tissues. Urocortin concentrations in synovial fluid were higher in RA patients (79.8 +/- 154 pg/ml) than in control patients (12.3 +/- 4.8 pg/ml; P less than or equal to 0.05). Urocortin immunoreactivity and mRNA signals were both detectedin synovial cells, lymphocytes, fibroblasts and macrophages. The number ofurocortin-positive cells in the synovium was significantly higher in RA (73.1 +/- 32.1 cells per high-power field) than in control (18.4 +/- 10.4 cells per high-power field) patients. In addition, both urocortin immunoreactivity and mRNA signals in the synovium reached maximum levels in the active stage of RA inflammation. Moreover, the number of immunoreactive urocortin-positive cells was significantly correlated with the urocortin concentration in synovial fluid (r = 0.705; P < 0.001) and with histologically defined local inflammatory activity (r = 0.641; P(0.001). The distribution and number of immunoreactive CRF-positive cells in synovial tissue were similar to those of urocortin-positive cells (r = 0.701; P < 0.001). Urocortin, CRF-RIand CRF-R2-alpha mRNAs detected by RT-PCR were expressed in in the synovium of 10/10, 10/10 and 2/10 RA patients respectively, but CRF-R2-beta was not expressed. Urocortin was actively synthesized in the synovium of RA patients. The present study suggests that urocortin may play an important role as an autocrine and/or paracrine regulator of synovial inflammation in RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:41:21