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Titolo:
Chemically defined protein-free in vitro culture of mammalian embryo does not restrict its developmental potential for differentiation of skin appendages
Autore:
Bulic-Jakus, F; Strahinic-Belovari, T; Maric, S; Jezek, D; Juric-Lekic, G; Vlahovic, M; Serman, D;
Indirizzi:
Univ Zagreb, Fac Med, Dept Biol, HR-10000 Zagreb, Croatia Univ Zagreb Zagreb Croatia HR-10000 Dept Biol, HR-10000 Zagreb, Croatia Univ Zagreb, Fac Med, Dept Histol & Embryol, HR-10000 Zagreb, Croatia UnivZagreb Zagreb Croatia HR-10000 & Embryol, HR-10000 Zagreb, Croatia Univ Osijek, Fac Med, Dept Histol & Embryol, Osijek, Croatia Univ Osijek Osijek Croatia Med, Dept Histol & Embryol, Osijek, Croatia Univ Osijek, Fac Med, Dept Biol, Osijek, Croatia Univ Osijek Osijek Croatia Osijek, Fac Med, Dept Biol, Osijek, Croatia
Titolo Testata:
CELLS TISSUES ORGANS
fascicolo: 2, volume: 169, anno: 2001,
pagine: 134 - 143
SICI:
1422-6405(2001)169:2<134:CDPIVC>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM-FREE CULTURE; RAT EGG-CYLINDERS; GROWTH-FACTORS; HAIR FOLLICLE; TRANSFERRIN RECEPTORS; EXPRESSION PATTERNS; DERMAL SHEATH; STEM-CELLS; IN-VITRO; MORPHOGENESIS;
Keywords:
skin appendages; culture, serum-free, in vitro; grafting; development; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Bulic-Jakus, F Univ Zagreb, Fac Med, Dept Biol, Salata 3, HR-10000 Zagreb,Croatia Univ Zagreb Salata 3 Zagreb Croatia HR-10000 greb, Croatia
Citazione:
F. Bulic-Jakus et al., "Chemically defined protein-free in vitro culture of mammalian embryo does not restrict its developmental potential for differentiation of skin appendages", CELLS T ORG, 169(2), 2001, pp. 134-143

Abstract

In a unique serum- and protein-free chemically defined in vitro culture model of postimplantation mammalian development the epidermis differentiates regularly, although the differentiation of other tissues is impaired due tothe lack of the serum. The present study in that model was done to estimate more carefully the deg ree of epidermal differentiation in defined media supplemented with some growth- or differentiation-stimulating substances. The main objective was to discover by grafting in vivo to the richer environment whether simple protein-free culture conditions restrict an inherent embryonic potential for differentiation of skin appendages. Embryonic parts of E9.5 gastrulating Fischer rat embryos were cultivated for 2 weeks in the protein-free Eagle's minimum essential medium supplemented with holotransferrin, apotransferrin, insulin and/or Na2SeO3 and in controls cultivated in protein-free medium or in serum-supplemented medium. In all experiments there was a high incidence of differentiation of the epidermis. A high level of epidermal differentiation was confirmed for the first time at the ultrastructural level. A well-differentiated cornified layer and cells connected with desmosomes containing keratohyaline masses and cytokeratin filaments were found. A strong immunohistochemical signal for the proliferating cell nuclear antigen was always detected in the basal layer of the epidermis showing that those cells were still able to proliferate. Finally, embryos precultivated for 1 or 2 weeks in the protein-free medium and media supplemented with apotransferrin or serum were grafted under the kidney capsule for an additional 2 weeks. It was discovered that even after spending 2 weeks in the simple protein-free medium in vitro, embryos retained their developmentalpotential for differentiation of skin appendages (hair and sebaceous glands). Copyright (C) 2001 S. Karger AG, Basel.

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Documento generato il 25/11/20 alle ore 18:56:34