Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cerebellar glia cells induce a correct laminar organization in chicken retinal reaggregates
Autore:
Willbold, E; Rothermel, A; Huhn, J; Reinicke, M; Layer, PG;
Indirizzi:
Tech Univ Darmstadt, Inst Zool, D-64287 Darmstadt, Germany Tech Univ Darmstadt Darmstadt Germany D-64287 D-64287 Darmstadt, Germany
Titolo Testata:
CELLS TISSUES ORGANS
fascicolo: 2, volume: 169, anno: 2001,
pagine: 104 - 112
SICI:
1422-6405(2001)169:2<104:CGCIAC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PIGMENTED EPITHELIUM; NEURAL RETINA; MULLER CELLS; IN-VITRO; INVITRO; GROWTH; REGENERATION; CULTURE; RABBIT;
Keywords:
retina; glia cells; lamination; rotation culture; tissue polarity; chicken;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Willbold, E Tech Univ Darmstadt, Inst Zool, Schnittspahnstr 3, D-64287 Darmstadt, Germany Tech Univ Darmstadt Schnittspahnstr 3 Darmstadt Germany D-64287
Citazione:
E. Willbold et al., "Cerebellar glia cells induce a correct laminar organization in chicken retinal reaggregates", CELLS T ORG, 169(2), 2001, pp. 104-112

Abstract

We investigated the functional role of glia cells during retinogenesis using the rotation culture system. Reaggregating cells from the embryonic chick retina have the unique capacity to reassemble into laminated cellular spheres. These spheres are composed of several compartments holding the constituents of many retinal layers in a topologically correct, yet inverse orientation. However, when these spheres are cultured in the presence of conditioned media derived from monolayers of cerebellar glia cells, the reassembling retinal cells behave totally differently. The anlage of the originally reversed lamina polarity is progressively transformed within a week into a sphere with a compound and correctly laminated orientation. Conditioned media from fibroblasts, other glia cells (except Muller cells) or a set of already characterized retinogenetic factors are not able to produce this dramatic transformation. Additionally, we were able to show that only retinal cells are able to respond with a reorganization process. Reaggregating cells from the chick cerebellum also form spheroids; however, neither in the presence of cerebellar glia cellderived conditioned medium nor their control counterparts are they able to reassemble histotypically. This indicates that cerebellar glia cells produce diffusible factors to which retinal cells can respond and that these factors can act as important determinants for the correct establishment of the retinal polarity. Since all types of laminar disorganization are of great clinical significance, the knowledge of factors which determine and sustain the normal retinal architecture are biomedicallyhighly relevant. Copyright (C) 2001S.KargerAG,Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:09:17