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Titolo:
Adeno-associated virus-mediated transfer of endothelial nitric oxide synthase gene inhibits protein synthesis of rat ventricular cardiomyocytes
Autore:
Maeda, Y; Ikeda, U; Oya, K; Shimpo, M; Ueno, S; Urabe, M; Kume, A; Monahan, J; Ozawa, K; Shimada, K;
Indirizzi:
Jichi Med Sch, Dept Cardiol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 Tochigi 3290498, Japan Jichi Med Sch, Ctr Mol Med, Div Genet Therapeut, Minami Kawachi, Tochigi 32904, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 32904 hi, Tochigi 32904, Japan Avigen Inc, Alameda, CA USA Avigen Inc Alameda CA USAAvigen Inc, Alameda, CA USA
Titolo Testata:
CARDIOVASCULAR DRUGS AND THERAPY
fascicolo: 1, volume: 15, anno: 2001,
pagine: 19 - 24
SICI:
0920-3206(2001)15:1<19:AVTOEN>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARDIAC MYOCYTES; SMOOTH-MUSCLE; AAV VECTORS; EXPRESSION; CELLS; EFFICIENT; BRAIN; PROLIFERATION; HYPERTENSION; HYPERTROPHY;
Keywords:
nitric oxide synthase; gene transfer; cardiac hypertrophy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, U Jichi Med Sch, Dept Cardiol, Minami Kawachi, Tochigi 3290498, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 3290498 290498, Japan
Citazione:
Y. Maeda et al., "Adeno-associated virus-mediated transfer of endothelial nitric oxide synthase gene inhibits protein synthesis of rat ventricular cardiomyocytes", CARDIO DRUG, 15(1), 2001, pp. 19-24

Abstract

We investigated whether nitric oxide (NO) synthase gene transfer could attenuate growth of cultured cardiac myocytes. First, we investigated the effects of exogenous NO and cGMP analog on protein synthesis of cultured neonatal rat cardiac myocytes. The NO donor 3-morpholino-sydnonimine-hydrochloride (SIN-1) and 8-bromo-cGMP caused concentration-dependent decreases in phenylephrine-stimulated incorporation of H-3-leucine into cardiac myocytes. Wethen transferred endothelial constitutive NO synthase (ecNOS) gene into cultured neonatal rat cardiac myocytes using adeno-associated virus (AAV) vectors. ecNOS gene transfer into cardiac myocytes induced 140 kD ecNOS protein expression and significantly increased cGMP contents of myocytes comparedwith control cells. ecNOS gene transfer inhibited H-3-leucine incorporation into cardiac myocytes in response to phenylephrine, which was significantly recovered in the presence of the NOS inhibitor N-G-monomethyl-L-arginineacetate. These results indicate that endogenously generated NO by ecNOS gene transfer using AAV vectors inhibits the alpha -adrenergic agonist-induced cardiac protein synthesis at least partially via cGMP production.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 02:11:59