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Titolo:
Corticosterone supplementation reduced selective protein kinase C isoform expression in the epidermis of adrenalectomized mice
Autore:
Birt, DF; Duysen, E; Wang, WQ; Yaktine, A;
Indirizzi:
Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA Iowa StateUniv Ames IA USA 50011 od Sci & Human Nutr, Ames, IA 50011 USA Univ Nebraska, Med Ctr, Eppley Inst Res Canc & Allied Dis, Omaha, NE 68198USA Univ Nebraska Omaha NE USA 68198 es Canc & Allied Dis, Omaha, NE 68198USA Univ Nebraska, Med Ctr, Dept Biochem & Mol Biol, Omaha, NE 68198 USA Univ Nebraska Omaha NE USA 68198 Biochem & Mol Biol, Omaha, NE 68198 USA
Titolo Testata:
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
fascicolo: 6, volume: 10, anno: 2001,
pagine: 679 - 685
SICI:
1055-9965(200106)10:6<679:CSRSPK>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SKIN TUMOR PROMOTION; DIETARY ENERGY RESTRICTION; GLUCOCORTICOID RECEPTOR; EPITHELIAL-CELLS; FOOD RESTRICTION; SENCAR MOUSE; INHIBITION; RAF-1; RAS; MODULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Birt, DF Iowa State Univ, Dept Food Sci & Human Nutr, 2312 Food Sci Bldg, Ames, IA 50011 USA Iowa State Univ 2312 Food Sci Bldg Ames IA USA 50011 IA 50011 USA
Citazione:
D.F. Birt et al., "Corticosterone supplementation reduced selective protein kinase C isoform expression in the epidermis of adrenalectomized mice", CANC EPID B, 10(6), 2001, pp. 679-685

Abstract

Previous research in this laboratory demonstrated elevated plasma corticosterone and reduced protein kinase C (PKC) activity and selective isoform expression in the epidermis of dietary energy-restricted mice. Because PKC isimplicated in skin carcinogenesis and because both energy restriction and glucocorticoid hormone inhibit skin carcinogenesis, the purpose of the present research was to determine whether the elevated glucocorticoid hormone in the energy-restricted mouse contributed to the changes in PKC protein expression, Two strategies were used to control corticosterone in adrenalectomized mice: (a) corticosterone-containing pellets were implanted in mice, and a dose response increase in corticosterone was observed with 5-, 10-, and35-mg corticosterone implants with average peak values of 68 +/- 22 ng/ml (P < 0.01); and (b) corticosterone was administered in the drinking water, and plasma corticosterone was elevated in a dose-dependent manner in mice killed at 6:00-6:30 p,m, (P < 0.01; peak values of 300-400 ng/ml). The expression of PKC alpha, PKC delta, and PKC is an element of protein were not consistently altered by corticosterone with the two strategies. PKC eta protein expression was elevated in the adrenalectomized mice administered 3 or 60 mug of corticosterone/ml in drinking water (P < 0.01). PKC xi protein expression was reduced by all doses of corticosterone in the implant or drinking water (P < 0.05), and a reduction of 41% was achieved with the mice administered 60 mug of corticosterone/ml in drinking water. In mice fed controlor energy-restricted diet, with or without adrenalectomy, PKC xi protein was reduced in sham-operated, energy-restricted mice in comparison with control diet, sham-operated mice (P < 0.02), whereas PKC xi protein was not significantly different between adrenalectomized control and adrenalectomized,energy-restricted mice. These data indicate that administration of corticosterone in drinking water most closely mimicked the circulating corticosterone and epidermal PKC changes observed in dietary energy restriction. Elevated plasma glucocorticoid levels in the dietary energy-restricted mouse maycontribute to the alteration of PKC protein levels in the epidermis.

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Documento generato il 23/01/20 alle ore 03:42:26