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Titolo:
Rapid flow-induced responses in endothelial cells
Autore:
Stamatas, GN; McIntire, LV;
Indirizzi:
Rice Univ, Inst Biosci & Bioengn, Cox Lab Biomed Engn, Houston, TX 77251 USA Rice Univ Houston TX USA 77251 Cox Lab Biomed Engn, Houston, TX 77251 USA
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 3, volume: 17, anno: 2001,
pagine: 383 - 402
SICI:
8756-7938(200105/06)17:3<383:RFRIEC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUID SHEAR-STRESS; NF-KAPPA-B; GENE-EXPRESSION; INTERMEDIATE FILAMENTS; SIGNAL-TRANSDUCTION; CALCIUM GRADIENTS; NUCLEAR CALCIUM; VASCULAR CELLS; CYTOSOLIC CA2+; STEADY FLOW;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
99
Recensione:
Indirizzi per estratti:
Indirizzo: McIntire, LV Rice Univ, Inst Biosci & Bioengn, Cox Lab Biomed Engn, POB 1892, Houston, TX 77251 USA Rice Univ POB 1892 Houston TX USA 77251 Houston, TX 77251 USA
Citazione:
G.N. Stamatas e L.V. McIntire, "Rapid flow-induced responses in endothelial cells", BIOTECH PR, 17(3), 2001, pp. 383-402

Abstract

Endothelial cells alter their morphology, growth rate, and metabolism in response to fluid shear stress. To study rapid flow-induced responses in the3D endothelial cell morphology and calcium distribution, coupled fluorescence microscopy with optical sectioning, digital imaging, and numerical deconvolution techniques have been utilized. Results demonstrate that within the first minutes of flow application nuclear calcium is increasing. In the same time frame whole cell height and nuclear height are reduced by about 1 mum. Whole cell height changes may facilitate reduction of shear stress gradients on the luminal surface, whereas nuclear structural changes may be important for modulating endothelial growth rate and metabolism. To study therole of the cytoskeleton in these responses, endothelial cells have been treated with specific disrupters (acrylamide, cytochalasin D, and colchicine) of each of the cytoskeleton elements (intermediate filaments, microfilaments, and microtubules, respectively). None of these compounds had any effect on the shear-induced calcium response. Cytochalasin D and acrylamide did not affect the shear-induced nuclear morphology changes. Colchicine, however, completely abrogated the response, indicating that microtubules may be implicated in force transmission from the plasma membrane to the nucleus. A pedagogical model based on tensegrity theory principles is presented that is consistent with the results on the 3D endothelial morphology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:21:43