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Titolo:
Identification of N-terminal autodigestion target site in subtilisin ALP I
Autore:
Maeda, H; Yamagata, Y; Ichishima, E; Nakajima, T;
Indirizzi:
Tohoku Univ, Grad Sch Agr Sci, Div Life Sci, Lab Mol Enzymol,Aoba Ku, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 Ku, Sendai, Miyagi 9818555, Japan Soka Univ, Grad Sch Engn, Dept Bioengn, Tokyo 1928577, Japan Soka Univ Tokyo Japan 1928577 h Engn, Dept Bioengn, Tokyo 1928577, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 5, volume: 65, anno: 2001,
pagine: 1255 - 1257
SICI:
0916-8451(200105)65:5<1255:IONATS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALKALOPHILIC BACILLUS SP;
Keywords:
serine protease; autodigestion; alkaline condition; alkaline stability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Maeda, H Tohoku Univ, Grad Sch Agr Sci, Div Life Sci, Lab Mol Enzymol,AobaKu, 1-1 Tsutsumidori Amamiyamachi, Sendai, Miyagi 9818555, Japan Tohoku Univ 1-1 Tsutsumidori Amamiyamachi Sendai Miyagi Japan 9818555
Citazione:
H. Maeda et al., "Identification of N-terminal autodigestion target site in subtilisin ALP I", BIOS BIOT B, 65(5), 2001, pp. 1255-1257

Abstract

Autodigestion of subtilisin ALP I (ALP I), secreted from the alkalophilic Bacillus sp. NKS-21 and its predicted amino acid sequence having about 60% identity with other alkaline subtilisins, was examined under alkaline conditions, At the alkaline pH of 12, ALP I was rapidly degraded, and almost no breakdown products were detectable. However, by incubating ALP I at 5 degreesC for an extended time, a couple of specific peptides (26.7 kDa and 25.6 kDa) were accumulated. Each of them was purified and amino acid sequences of these fragments were found. Both peptides appeared to start at Gly-19 of the mature sequence of ALP I.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/18 alle ore 08:46:59