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Titolo:
Confocal microscopic study of GABA(A) receptors in Xenopus oocytes after rat brain mRNA injection: modulation by tyrosine kinase activity
Autore:
Balduzzi, R; Cupello, A; Diaspro, A; Ramoino, P; Robello, M;
Indirizzi:
Univ Genoa, Unita INFM, Dipartimento Fis, I-16146 Genoa, Italy Univ GenoaGenoa Italy I-16146 M, Dipartimento Fis, I-16146 Genoa, Italy CNR, Ctr Neurofisiol Cerebrale, Genoa, Italy CNR Genoa ItalyCNR, Ctr Neurofisiol Cerebrale, Genoa, Italy Univ Genoa, Dip Te Ris, Genoa, Italy Univ Genoa Genoa ItalyUniv Genoa, Dip Te Ris, Genoa, Italy
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
fascicolo: 1-2, volume: 1539, anno: 2001,
pagine: 93 - 100
SICI:
0167-4889(20010528)1539:1-2<93:CMSOGR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETYLCHOLINE-RECEPTOR; MESSENGER-RNA; SUBUNIT COMPOSITION; MUSCLE-CELLS; PHOSPHORYLATION; ACTIVATION; PHOSPHATASE; PINHOLE; ACID;
Keywords:
GABA(A) receptor; Xenopus oocyte; immunocytochemistry; protein tyrosine kinase; genistein; phosphatase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Robello, M Univ Genoa, Unita INFM, Dipartimento Fis, Via Dodecaneso 33, I-16146 Genoa, Italy Univ Genoa Via Dodecaneso 33 Genoa Italy I-16146 Genoa, Italy
Citazione:
R. Balduzzi et al., "Confocal microscopic study of GABA(A) receptors in Xenopus oocytes after rat brain mRNA injection: modulation by tyrosine kinase activity", BBA-MOL CEL, 1539(1-2), 2001, pp. 93-100

Abstract

The expression of GABAA receptors in Xenopus oocytes injected with rat brain mRNA was studied by immunocytochemistry and evaluation of the distribution of fluorescent probes at the confocal microscope. The beta (2/3) subunitdistributed exclusively on the membrane at the animal pole of the oocytes. Treatment of oocytes for 20 min with the protein tyrosine kinase inhibitorgenistein, 200 muM, resulted in a lower presence of GABA(A) receptors on the membrane. The inactive genistein analogue daidzein, 200 muM, had no effect even with a 30 min treatment. Alkaline phosphatase but not a protein tyrosine phosphatase, when injected into oocytes, reduced GABA receptor membrane expression. The data indicate that protein tyrosine phosphorylation modulates the expression on the plasma membrane of presynthesized GABAA receptors. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 13:19:13