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Titolo:
Chemometric optimisation of parameters for biocatalytic reduction of copper ion by a crude enzyme lysate of Saccharomyces cerevisiae grown under catabolic repression conditions
Autore:
Chandran, CB; Subramanian, TV; Felse, PA;
Indirizzi:
Anna Univ, Dept Chem Engn, Chennai, India Anna Univ Chennai IndiaAnna Univ, Dept Chem Engn, Chennai, India Indian Inst Technol, Dept Chem Engn, Biotechnol Res Ctr, Chennai, India Indian Inst Technol Chennai India n, Biotechnol Res Ctr, Chennai, India
Titolo Testata:
BIOCHEMICAL ENGINEERING JOURNAL
fascicolo: 1, volume: 8, anno: 2001,
pagine: 31 - 37
SICI:
1369-703X(200107)8:1<31:COOPFB>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Keywords:
biocatalytic reduction; removal of copper ions; cytochrome P-450; response surface methodology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Chandran, CB Univ Ulster, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry,North Ireland Univ Ulster Coleraine Londonderry North Ireland BT52 1SA and
Citazione:
C.B. Chandran et al., "Chemometric optimisation of parameters for biocatalytic reduction of copper ion by a crude enzyme lysate of Saccharomyces cerevisiae grown under catabolic repression conditions", BIOCH ENG J, 8(1), 2001, pp. 31-37

Abstract

The capacity of the microbes to reduce the metal has been demonstrated. The immobilised induced microbes with toxic chemical CuCl2 was used to reducethe Cu ions as elemental metal and by using the response surface methodology the parameters such as the inducer concentration, the time of inducer addition which are concerned with the growth and formation of specific enzymes and the initial substrate concentration, initial pH of the substrate solution and the time of reaction which are concerned with the biocatalytic reduction of the metal ions were optimised for maximum reduction. The elemental copper reduced and removed experimentally from its ionic state at the optimum conditions was 54.82 ppm. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 04:46:28