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Titolo:
An in vivo approach to identifying sequence context of 8-oxoguanine mutagenesis
Autore:
Watanabe, T; Nunoshiba, T; Kawata, N; Yamamoto, K;
Indirizzi:
Tohoku Univ, Inst Mol Life Sci, Grad Sch Life Sci, Sendai, Miyagi 9808578,Japan Tohoku Univ Sendai Miyagi Japan 9808578 Sci, Sendai, Miyagi 9808578,Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 284, anno: 2001,
pagine: 179 - 184
SICI:
0006-291X(20010601)284:1<179:AIVATI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLI SUPF GENE; ESCHERICHIA-COLI; MUTATIONAL SPECIFICITY; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; G-C->C-G TRANSVERSIONS; FERROUS ION; DNA; SPECTRA; DAMAGE; SYSTEM;
Keywords:
mutation hotspot; 7,8-dihydro-8-oxoguanine; supF gene; mutM; mutY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Yamamoto, K Tohoku Univ, Inst Mol Life Sci, Grad Sch Life Sci, Sendai, Miyagi 9808578,Japan Tohoku Univ Sendai Miyagi Japan 9808578 Miyagi 9808578,Japan
Citazione:
T. Watanabe et al., "An in vivo approach to identifying sequence context of 8-oxoguanine mutagenesis", BIOC BIOP R, 284(1), 2001, pp. 179-184

Abstract

Base substitution mutations are not distributed randomly in that most are located at a few specific hotspots sites. We have been studying 7,8-dihydro-8-oxoguanine mutagenesis in Escherichia coli in the supF gene carried in aplasmid. Among hotspots, guanine within the 5 ' -AGA-3 ' located in the anticodon site was susceptible to the induction of G:C -->T:A transversion. In this study, we constructed variants of the supF gene in which the hotspot5 ' -AGA-3 ' was modifiied to 5 ' -AGT-3 ', 5 ' -AGG-3 ' and 5 ' -AGC-3 ' to determine the influence of 3 ' neighboring base on G:C -->T:A mutationalactivity. Using these variant supF genes propagated in a 7,8-dihydro-8-oxoguanine repair-deficient host, we found that guanine within 5 ' -AGA-3 ' and 5 ' -AGG-3 ' produce G:C -->T:A, but guanine within 5 ' -AGT-3 ' and 5 ' -AGC-3 ' reduce the formation of G:C -->T:A. These changes were thus due tothe effect of sequence context on the efficiency of mutation formation at the sites of 7,8-dihydro-8-oxoguanine. We also observed a longer range base-pair effect on hotspot formation. (C) 2001 Academic Press.

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Documento generato il 29/05/20 alle ore 13:20:33