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Titolo:
Clinical significance of K-ras mutations in intraoperative tumor drainage blood from patients with colorectal carcinoma
Autore:
Etoh, T; Ueo, H; Inoue, H; Sato, K; Utsunomiya, T; Barnard, GF; Kitano, S; Mori, M;
Indirizzi:
Kyushu Univ, Med Inst Bioregulat, Dept Surg, Beppu, Oita 8740838, Japan Kyushu Univ Beppu Oita Japan 8740838 ept Surg, Beppu, Oita 8740838, Japan Oita Prefectural Hosp, Dept Surg, Oita, Japan Oita Prefectural Hosp OitaJapan efectural Hosp, Dept Surg, Oita, Japan Univ Massachusetts, Sch Med, Div Digest Dis & Nutr, Worcester, MA 01605 USA Univ Massachusetts Worcester MA USA 01605 & Nutr, Worcester, MA 01605 USA Oita Med Univ, Fac Med, Dept Surg 1, Oita, Japan Oita Med Univ Oita Japan ta Med Univ, Fac Med, Dept Surg 1, Oita, Japan
Titolo Testata:
ANNALS OF SURGICAL ONCOLOGY
fascicolo: 5, volume: 8, anno: 2001,
pagine: 407 - 412
SICI:
1068-9265(200106)8:5<407:CSOKMI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; ALLELE-SPECIFIC AMPLIFICATION; MOLECULAR-DETECTION; PERIPHERAL-BLOOD; CANCER PATIENTS; CARCINOEMBRYONIC ANTIGEN; LYMPH-NODES; POINT MUTATIONS; GENE-MUTATIONS; CELLS;
Keywords:
K-ras mutation; MACS system; prognosis; drainage blood; colorectal carcinoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Mori, M Kyushu Univ, Med Inst Bioregulat, Dept Surg, 4546 Tsurumihara, Beppu, Oita8740838, Japan Kyushu Univ 4546 Tsurumihara Beppu Oita Japan 8740838 40838, Japan
Citazione:
T. Etoh et al., "Clinical significance of K-ras mutations in intraoperative tumor drainage blood from patients with colorectal carcinoma", ANN SURG O, 8(5), 2001, pp. 407-412

Abstract

Background: Recurrent and metastatic carcinoma of the colorectum remains amajor problem. This may be ascribed to the presence of micrometastasis at diagnosis. The purpose of this study was to analyze prospectively the clinical value of detecting K-ras mutations in the perioperative circulating blood from patients with colorectal carcinoma. Methods: Twenty-four patients whose tumor carried mutations in codon 12 ofthe K-ras gene were studied for the presence of cancer cells in perioperative blood samples, in particular, tumor drainage samples. A detection assayusing CD45 immunomagnetic separation plus nested mutant allele specific amplification (MASA) was performed. Results: K-ras mutations in CD45 negative cells in tumor drainage blood were detected in 7 (29.2%) of 24 patients. There was no significant relationship between the presence of a K-ras mutation and clinicopathological features. Four (57.1%) of the seven patients with a positive K-ras mutation in drainage blood had early recurrent disease. Of the 17 patients with no K-ras mutation, none developed metastatic disease. The recurrence rate of the K-ras mutation positive group was higher than that of the K-ras mutation negative group (P < .01). There was a significant difference, regarding prognosis, between K-ras mutation positive and negative groups (P < .01). Conclusions: This preliminary study demonstrates that the detection of circulating cancer cells in the tumor drainage blood by our new assay system may provide a predictor of recurrence and metastasis of colorectal cancer.

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Documento generato il 23/01/20 alle ore 03:36:12