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Titolo:
Comparative phospholipid analogue distributions of Porphyromonas gingivalis isolated from cats in Australia and the USA
Autore:
Korachi, M; Love, D; Goldstein, EJ; Citron, DM; Blinkhorn, AS; Boote, V; Drucker, DB;
Indirizzi:
Univ Manchester, Sch Dent, Oral Microbiol Lab, Manchester M15 6FH, Lancs, England Univ Manchester Manchester Lancs England M15 6FH M15 6FH, Lancs, England Univ Sydney, Dept Vet Anat & Pathol, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 Pathol, Sydney, NSW 2006, Australia Univ Calif Los Angeles, Santa Monica Med Ctr, RM Aiden Res Lab, Santa Monica, CA 90404 USA Univ Calif Los Angeles Santa Monica CA USA 90404 nta Monica, CA 90404 USA Univ Manchester, Dept Chem, Mass Spectrometry Lab, Manchester M13 9PL, Lancs, England Univ Manchester Manchester Lancs England M13 9PL M13 9PL, Lancs, England
Titolo Testata:
VETERINARY MICROBIOLOGY
fascicolo: 2, volume: 81, anno: 2001,
pagine: 153 - 163
SICI:
0378-1135(20010726)81:2<153:CPADOP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOMBARDMENT MASS-SPECTROMETRY; GRAM-NEGATIVE ANAEROBES; BACTEROIDES-GINGIVALIS; PREVOTELLA; CLASSIFICATION; STRAINS;
Keywords:
Porphyromonas gingivalis; phospholipids; FAB-MS; cat; dog;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Drucker, DB Univ Manchester, Sch Dent, Oral Microbiol Lab, Higher Cambridge St, Manchester M15 6FH, Lancs, England Univ Manchester Higher Cambridge St Manchester Lancs England M15 6FH
Citazione:
M. Korachi et al., "Comparative phospholipid analogue distributions of Porphyromonas gingivalis isolated from cats in Australia and the USA", VET MICROB, 81(2), 2001, pp. 153-163

Abstract

DNA-DNA homology measurements and phospholipid (PL) analogue profiling have shown heterogeneity of Porphyromonas gingivalis, The aim of this study was to determine whether there were differences between cat strains of P. gingivalis from Australia and USA with respect to PL analogue distribution. Lipids were extracted with chloroform-methanol and examined by fast atom bombardment-mass spectrometry (FAB-MS) in negative-ion mode, using published methods. For PL analogues, the major anions included those with mass-to-charge (m/z) = 634, 648, 662, 705, 932, 946 and 960, respectively, correspondingto expected presence of PE (28:0), PE (29:0), PE (30:0), PG (32:1), and three unknown homologues of a glycero-phospholipid with a single nitrogen. Analyses were compared to calculate a matrix of Pearson coefficients of linear correlation from which a dendrogram was produced of strains clustered by single linkage. One cluster was comprised solely of Australian cat-to-cat bite isolates and a second duster included exclusively USA cat- and dog-to-human bite isolates except for one Australian cat-to-cat bite isolate (VPB 5089). The US cluster included three outliers, one of which was the Australian cat isolate VPB 5089. The human type strain (ATCC 33277) was quite remote from all dog and cat strains. It was shown that P: gingivalis human and non-human animal isolates have distinct PL analogue profiles from each other. Furthermore, the cat strains from the USA and those from Australia showedquantitative differences in polar lipid profiles that correlated largely with country of isolation. (C) 2001 Elsevier Science B.V. AH rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 15:24:25