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Titolo:
Gonadotropin-releasing hormone receptor expression in the human prostate
Autore:
Tieva, A; Stattin, P; Wikstrom, P; Bergh, A; Damber, JE;
Indirizzi:
Sahlgrens Univ Hosp, Dept Urol, Inst Surg Sci, S-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden S-41345 S-41345 Gothenburg, Sweden Umea Univ, Inst Surg & Perioperat Sci Urol & Androl, S-90185 Umea, Sweden Umea Univ Umea Sweden S-90185 at Sci Urol & Androl, S-90185 Umea, Sweden Umea Univ, Inst Med Biosci, S-90185 Umea, Sweden Umea Univ Umea Sweden S-90185 niv, Inst Med Biosci, S-90185 Umea, Sweden
Titolo Testata:
PROSTATE
fascicolo: 4, volume: 47, anno: 2001,
pagine: 276 - 284
SICI:
0270-4137(20010601)47:4<276:GHREIT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANCER CELL-LINES; TARGETED CYTOTOXIC ANALOG; MESSENGER-RNA EXPRESSION; RH ANTAGONIST CETRORELIX; EPIDERMAL GROWTH-FACTOR; HIGH-AFFINITY BINDING; NUDE-MICE; GENE-EXPRESSION; LH-RH; GNRH RECEPTOR;
Keywords:
GnRH-R; human prostate; prostate cancer; competitive RT-PCR; immunohistochemistry (IHC);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Damber, JE Sahlgrens Univ Hosp, Dept Urol, Inst Surg Sci, S-41345 Gothenburg, Sweden Sahlgrens Univ Hosp Gothenburg Sweden S-41345 henburg, Sweden
Citazione:
A. Tieva et al., "Gonadotropin-releasing hormone receptor expression in the human prostate", PROSTATE, 47(4), 2001, pp. 276-284

Abstract

BACKGROUND. Inhibitory effects of gonadotropin-releasing hormone (GnRH) analogs on prostate cancer cell proliferation, both in vivo and in vitro, indicate the presence of specific binding sites for GnRH on prostate cancer cells. To investigate this issue further, we examined the expression of GnRH receptor (GnRH-R) mRNA and protein in human prostate biopsies as well as inother extrapituitary tissues. METHOD. The relative quantity of GnRH-R mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC) in human prostate biopsies. Extrapituitary GnRH-R levels were determined by a semiquantitative PCR reaction. RESULTS. Using PCR, a relatively high expression level of GnRH-R mRNA was found in prostate tumor tissue followed by normal prostate, thymus, and kidney expression levels. The levels showed by heart, brain, placenta, lung, liver, skeletal muscle, pancreas, colon, ovary, small intestine, spleen, andtestis were low but detectable, whereas peripheral blood leukocyte showed no demonstrable product. GnRH-R immunoreactivity was localised in both luminal and basal epithelial cells in benign and malignant prostate tissue, andGnRH-R were also observed in intraprostatic lymphocytes. The relative GnRH-R mRNA levels in prostate biopsies from 16 patients showed a wide range ofindividual differences, but these differences were not related to histological grade. Castration therapy did not significantly influence GnRH-R mRNA expression in normal and malignant prostate tissue. CONCLUSION. These results suggest that epithelial cells and infiltrating lymphocytes are targets for GnRH action in the human prostate. Comparative data show relatively high GnRH-R expression in human prostate tissue compared to other human tissues. (C) 2001 Wiley-Liss,Inc.

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Documento generato il 03/07/20 alle ore 01:18:31