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Titolo:
The role of the ABC transporter gene PMR1 in demethylation inhibitor resistance in Penicillium digitatum
Autore:
Hamamoto, H; Nawata, O; Hasegawa, K; Nakaune, R; Lee, YJ; Makizumi, Y; Akutsu, K; Hibi, T;
Indirizzi:
Univ Tokyo, Dept Agr & Environm Biol, Bunkyo Ku, Tokyo 1138657, Japan UnivTokyo Tokyo Japan 1138657 onm Biol, Bunkyo Ku, Tokyo 1138657, Japan BRAIN, Ohmiya, Saitama 3310044, Japan BRAIN Ohmiya Saitama Japan 3310044BRAIN, Ohmiya, Saitama 3310044, Japan Natl Inst Fruit Tree Sci, Akitsu, Hiroshima 7292494, Japan Natl Inst FruitTree Sci Akitsu Hiroshima Japan 7292494 ma 7292494, Japan Ibaraki Univ, Fac Agr, Ami, Ibaraki 3000393, Japan Ibaraki Univ Ami Ibaraki Japan 3000393 c Agr, Ami, Ibaraki 3000393, Japan
Titolo Testata:
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
fascicolo: 1, volume: 70, anno: 2001,
pagine: 19 - 26
SICI:
0048-3575(200105)70:1<19:TROTAT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIDRUG-RESISTANCE; TRANSCRIPTION; FUNGUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Hamamoto, H Univ Tokyo, Dept Agr & Environm Biol, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 nkyo Ku, Tokyo 1138657, Japan
Citazione:
H. Hamamoto et al., "The role of the ABC transporter gene PMR1 in demethylation inhibitor resistance in Penicillium digitatum", PEST BIOCH, 70(1), 2001, pp. 19-26

Abstract

In a previous study, we showed that disruption of the Penicillium digitatum ABC transporter gene PMR1 in a demethylation inhibitor (DMI)-resistant strain decreases its DMI resistance and that the level of constitutive expression of PMR1 in the DMI-resistant strains was slightly higher than that in the sensitive strains. In this study, we carried out the following three experiments to investigate the specific role of PMR1 in DMI resistance: (i) disruption of PMR1 in a DMI-sensitive strain, (ii) sequencing of the PMR1 genes from DMI-sensitive and -resistant strains, and (iii) introduction of PMR1 under the control of a strong promoter or an intact inducible promoter into a PMR1-disrupted strain and into a DMI-sensitive strain. Our findings suggest that a PMR1-based toxicant efflux mechanism for DMI resistance is present in both the DMI-sensitive and the DMI-resistant strains, and it mightplay a role in determining the baseline resistance of this fungus. A slight difference in PMR1 expression is not considered to be the factor that determines the difference in DMI resistance between the sensitive strains and the resistant strains. (C) 2001 Academic Press.

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Documento generato il 07/07/20 alle ore 12:19:31