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Titolo:
Correlation of binding sites for diisopropyl phosphorofluoridate with cholinesterase and neuropathy target esterase in membrane and cytosol preparations from hen
Autore:
Kamata, R; Saito, SY; Suzuki, T; Takewaki, T; Kobayashi, H;
Indirizzi:
Iwate Univ, Fac Agr, Dept Vet Pharmacol, Morioka, Iwate 0208550, Japan Iwate Univ Morioka Iwate Japan 0208550 col, Morioka, Iwate 0208550, Japan Tohoku Univ, Fac Pharmaceut Sci, Dept Pharmaceut Mol Biol, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 ol, Sendai, Miyagi 9808578, Japan Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan Gifu Univ Gifu Japan 5011193 nited Grad Sch Vet Sci, Gifu 5011193, Japan
Titolo Testata:
NEUROTOXICOLOGY
fascicolo: 2, volume: 22, anno: 2001,
pagine: 203 - 214
SICI:
0161-813X(200104)22:2<203:COBSFD>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE; INDUCED DELAYED NEUROTOXICITY; MESSENGER-RNA EXPRESSION; (DFP)-TREATED HEN; ORGANOPHOSPHORUS COMPOUNDS; BRAIN SUPERNATANT; SPINAL-CORD; PHOSPHORYLATION; TOXICITY; ASSAY;
Keywords:
organophosphorus induced delayed neurotoxicity (OPIDN); diisopropyl phosphorofluoridate (DFP); cytosolic protein; hen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Kamata, R Iwate Univ, Fac Agr, Dept Vet Pharmacol, 3-18-8 Ueda, Morioka, Iwate 0208550, Japan Iwate Univ 3-18-8 Ueda Morioka Iwate Japan 0208550 208550, Japan
Citazione:
R. Kamata et al., "Correlation of binding sites for diisopropyl phosphorofluoridate with cholinesterase and neuropathy target esterase in membrane and cytosol preparations from hen", NEUROTOXICO, 22(2), 2001, pp. 203-214

Abstract

To find new putative target(s) for organophosphorus induced delayed neurotoxicity (OPIDN), we investigated the biochemical and pharmacological characteristics of [H-3]diisopropyl phosphorofluoridate (DFP) binding to membraneand c:cytosol preparations from the brain and spinal cord of hens. Specific [H-3]DFP binding was determined by subtracting non-specific binding from total binding. The binding sites of [H-3]DFP, an organophosphate that induces OPIDN, were found not only on membrane but also in cytosol. Reduction ofsubsequent ex vivo specific [H-3]DFP binding by in vivo pretreatment with unlabeled DFP was found in cytosol, not membrane. The reduced binding lasted to the onset of OPIDN, especially in spinal cord. These results suggest that the specific DFP binding sites in cytosol, rather than on membrane, arethe most important with regard to the initiation of OPIDN. Inhibitors of cholinesterase (ChE) and neuropathy target esterase (NTE) other than DFP didnos affect specific [3H]DFP binding to either membranes or cytosol in vivo. Additionally, inhibition of the activities of these esterases by these compounds was not consistent with either the degree of inhibition of the [H-3]DFP binding or a time-dependent manner of OPIDN. These results suggest that DFP binding site(s) involved in the initiation of OPIDN may be different from the active sites of ChE and NTE. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 09:04:44