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Titolo:
A comparative study of binding sites for diisopropyl phosphorofluoridate in membrane and cytosol preparations from spinal cord and brain of hens
Autore:
Kamata, R; Saito, SY; Suzuki, T; Takewaki, T; Kofujita, H; Ota, M; Kobayashi, H;
Indirizzi:
Iwate Univ, Fac Agr, Dept Vet Pharmacol, Morioka, Iwate 0208550, Japan Iwate Univ Morioka Iwate Japan 0208550 col, Morioka, Iwate 0208550, Japan Tohoku Univ, Fac Pharmaceut Sci, Dept Pharmaceut Mol Biol, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 ol, Sendai, Miyagi 9808578, Japan Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan Gifu Univ Gifu Japan 5011193 nited Grad Sch Vet Sci, Gifu 5011193, Japan Iwate Univ, Fac Agr, Dept Wood Sci & Technol, Morioka, Iwate 0208550, Japan Iwate Univ Morioka Iwate Japan 0208550 nol, Morioka, Iwate 0208550, Japan
Titolo Testata:
NEUROTOXICOLOGY
fascicolo: 2, volume: 22, anno: 2001,
pagine: 191 - 202
SICI:
0161-813X(200104)22:2<191:ACSOBS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROPATHY TARGET ESTERASE; INDUCED DELAYED NEUROTOXICITY; PHENYL VALERATE ESTERASES; ORGANOPHOSPHORUS; ACETYLCHOLINESTERASE; RECEPTORS; ASSAY; POLYNEUROPATHY; CHLORPYRIFOS; INHIBITORS;
Keywords:
organophosphorus induced delayed neurotoxicity (OPIDN); diisopropyl phosphorofluoridate (DFP); cytosolic protein; hen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kamata, R Iwate Univ, Fac Agr, Dept Vet Pharmacol, 3-18-8 Ueda, Morioka, Iwate 0208550, Japan Iwate Univ 3-18-8 Ueda Morioka Iwate Japan 0208550 208550, Japan
Citazione:
R. Kamata et al., "A comparative study of binding sites for diisopropyl phosphorofluoridate in membrane and cytosol preparations from spinal cord and brain of hens", NEUROTOXICO, 22(2), 2001, pp. 191-202

Abstract

Biochemical events in the initiation of organophosphorus induced delayed neurotoxicity (OPIDN) are not well understood To find new putative target(s)for OPIDN, rye investigated the biochemical and pharmacological characteristics of [H-3]diisopropyl phosphorofluoridate (DFP) binding to membrane andcytosol preparations from the brain and spinal cold of hens in vitro. [H-3]DFP binding to both preparations was determined by the specific binding obtained by subtracting non-specific binding from total binding. The specificbinding sites of [H-3]DFP were found not only on membrane but also in cytosol. K-d values were higher and B-max values were lower in cytosol than in membrane. Moreover; the Kd values in both membrane and cytosol preparationsfrom spinal cord were lower than those of brain. The B-max values in membrane and cytosol were similar between brain and spinal cord. The specific binding to both preparations was markedly displaced by unlabeled DFP. The specific binding of DFP to the membrane was highly or partly displaced by organophosphorus compounds (OPs) or a carbamate, respectively. However both theOPs and the carbanmate had considerably weaker blocking effects on the specific binding of DFP to cytosol. Noire of the compounds known to interact with neuropathy target esterase (NTE) had a strong blocking effect on the specific binding of DFP to either membrane or cytosol. These results show that the specific binding of DFP to the membrane may be binding with cholinesterase (ChE). However; cytosol, especially in spinal cord, may have DFP binding sites other than ChE and NTE. (C) 2001 Elsevier Science Inc. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:10:57