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Titolo:
Characterization of a brain-related serine protease, neurosin (human kaillikrein 6), in human cerebrospinal fluid
Autore:
Okui, A; Kominami, K; Uemura, H; Mitsui, S; Yamaguchi, N;
Indirizzi:
Kyoto Prefectural Univ Med, Res Inst Geriatr, Dept Cell Biol, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 iol, Kyoto 6028566, Japan Fuso Pharmaceut Inc, Res & Dev Ctr, Osaka, Japan Fuso Pharmaceut Inc Osaka Japan maceut Inc, Res & Dev Ctr, Osaka, Japan
Titolo Testata:
NEUROREPORT
fascicolo: 7, volume: 12, anno: 2001,
pagine: 1345 - 1350
SICI:
0959-4965(20010525)12:7<1345:COABSP>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-PLASMINOGEN ACTIVATOR; ALZHEIMERS-DISEASE; ZYME/PROTEASE-M/NEUROSIN; NEURITE OUTGROWTH; MOLECULAR-CLONING; THROMBIN; NEXIN; CELLS; INHIBITOR; PRECURSOR;
Keywords:
cerebrospinal fluid; human kallikrein 6; human kallikreins; neurosin; pro-enzyme; serine protease; zyme/protease M;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaguchi, N Kyoto Prefectural Univ Med, Res Inst Geriatr, Dept Cell Biol,Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 28566, Japan
Citazione:
A. Okui et al., "Characterization of a brain-related serine protease, neurosin (human kaillikrein 6), in human cerebrospinal fluid", NEUROREPORT, 12(7), 2001, pp. 1345-1350

Abstract

Neurosin (also known as zyme or protease M) is a trypsin-like serine protease dominantly expressed in the human brain. According to the official nomenclature, this gene is now designated as human kallikrein 6 (KLK6) and the protein is designated hK6. To investigate the metabolism of neurosin in human brain, neurosin contained in the human cerebrospinal fluid (CSF) was analyzed. Neurosin was detected in the all CSFs tested by Western blot analysis using an anti-neurosin monoclonal antibody. We purified neurosin from CSF(CSF-neurosin) using an immunoaffinity chromatography and an anion-exchange chromatography. SDS-PAGE revealed that the purified protein has a relative mot. mass (Mr) of 25,000 Da. The observed sequence of the N-terminal amino acids, Glu-Glu-Gln-Asn-Lys, of the purified CSF-neurosin was identical tothe sequence of N-terminal of the pro-enzyme form, which is presumed to have no enzyme activity. CSF-neurosin neither showed any enzyme activity to Boc-Ph-Ser-Arg-4-methylcoumaryl-7-amide, which is known to be degraded by the mature neurosin, nor cleaved gelatin. To confirm that the major portion of CSF-neurosin is present in the pro-enzyme form, Western blot analysis using antibodies specific to the pro- or mature enzyme was carried out. The antibody against the mature neurosin fragment did not react with CSF-neurosin. Only the antibody against the pro-enzyme fragment detected CSF-neurosin. Thus, our results suggest that neurosin is present as an inactive pro-enzyme in the human CSF. NeuroReport 12:1345-1350. (C) 2001 Lippincott Williams & Wilkins.

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Documento generato il 25/11/20 alle ore 01:11:37