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Titolo:
Characterisation of neuropeptide Y receptor subtypes by synthetic NPY analogues and by anti-receptor antibodies
Autore:
Eckard, CP; Cabrele, C; Wieland, HA; Beck-Sickinger, AG;
Indirizzi:
Swiss Fed Inst Technol, ETH Zurich, Dept Appl Biosci, CH-8057 Zurich, Switzerland Swiss Fed Inst Technol Zurich Switzerland CH-8057 57 Zurich, Switzerland Boehringer Ingelheim Pharma AG, Div Preclin Res, D-88397 Biberach, GermanyBoehringer Ingelheim Pharma AG Biberach Germany D-88397 iberach, Germany Univ Leipzig, Inst Biochem, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 t Biochem, D-04103 Leipzig, Germany
Titolo Testata:
MOLECULES
fascicolo: 5, volume: 6, anno: 2001,
pagine: 448 - 467
SICI:
1420-3049(200105)6:5<448:CONYRS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-COUPLED RECEPTORS; PEPTIDE-YY; PANCREATIC-POLYPEPTIDE; FUNCTIONAL EXPRESSION; Y-2 RECEPTORS; HUMAN BRAIN; CLONING; BINDING; RAT; DESENSITIZATION;
Keywords:
neuropeptide Y; NPY analogues; anti-receptor antibodies; NPY receptor; subtype selectivity; ligand-binding site;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Beck-Sickinger, AG Swiss Fed Inst Technol, ETH Zurich, Dept Appl Biosci, Winterthurer Str 190, CH-8057 Zurich, Switzerland Swiss Fed Inst Technol Winterthurer Str 190 Zurich Switzerland CH-8057
Citazione:
C.P. Eckard et al., "Characterisation of neuropeptide Y receptor subtypes by synthetic NPY analogues and by anti-receptor antibodies", MOLECULES, 6(5), 2001, pp. 448-467

Abstract

Neuropeptide Y (NPY), a 36-mer neuromodulator, binds to the receptors Y-1,Y-2, Y-4 and Y-5 with nanomolar affinity. They all belong to the rhodopsin-like G-protein coupled, seven transmembrane helix spanning receptors. In this study, Ala-substituted and centrally truncated NPY analogues were compared with respect to affinity to the Y-receptors. Furthermore, antibodies against the second (E2) and the third (E3) extracellular loop of NPY Y-1-, Y-2- and Y-5-receptor subtypes were raised and affinity to intact cells was tested by immunofluorescence assays. Both methods were applied in order to receive subtype selective tools and to characterise ligand binding. The analogues [A(13)]-pNPY and [A(27)]-pNPY showed subtype selectivity for the Yz-receptor. Sera against the E2 loop of the Y-1-receptor and against the E2 loop of the Y-2-receptor were subtype selective. Two antibodies against the Y-5 E2 and E3 loop recognised the Y-5- and Y-2-receptor subtypes. In combination, these sera are able to distinguish between the Y-1-, Y-2-, and Ys-receptor subtypes. The analogues and antibodies represent valuable tools to distinguish NPY receptors on membranes and intact cells.

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Documento generato il 29/03/20 alle ore 01:34:37