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Titolo:
Novel wasp toxin discriminates between neuronal and cardiac sodium channels
Autore:
Kinoshita, E; Maejima, H; Yamaoka, K; Konno, K; Kawai, N; Shimizu, E; Yokote, S; Nakayama, H; Seyama, I;
Indirizzi:
Hiroshima Univ, Sch Med, Dept Physiol, Hiroshima 7348551, Japan Hiroshima Univ Hiroshima Japan 7348551 Physiol, Hiroshima 7348551, Japan Hiroshima Univ, Sch Med, Inst Hlth Sci, Hiroshima 7348551, Japan HiroshimaUniv Hiroshima Japan 7348551 lth Sci, Hiroshima 7348551, Japan Sao Paulo State Univ, Inst Biosci Rio Claro, Sao Paulo, Brazil Sao Paulo State Univ Sao Paulo Brazil osci Rio Claro, Sao Paulo, Brazil Jichi Med Sch, Dept Physiol, Minami Kawachi, Tochigi 32904, Japan Jichi Med Sch Minami Kawachi Tochigi Japan 32904 hi, Tochigi 32904, Japan Kumamoto Univ, Fac Pharmaceut Sci, Dept Biofunct Chem, Kumamoto 862, JapanKumamoto Univ Kumamoto Japan 862 Dept Biofunct Chem, Kumamoto 862, Japan
Titolo Testata:
MOLECULAR PHARMACOLOGY
fascicolo: 6, volume: 59, anno: 2001,
pagine: 1457 - 1463
SICI:
0026-895X(200106)59:6<1457:NWTDBN>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-ANEMONE TOXIN; ALPHA-SCORPION TOXIN; ANTHOPLEURIN-B; NA+-CHANNELS; MOLECULAR DETERMINANTS; CATIONIC RESIDUES; SOLITARY WASP; RECEPTOR-SITE; HIGH-AFFINITY; INACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Seyama, I Hiroshima Univ, Sch Med, Dept Physiol, Kasumi 1-2-3, Hiroshima 7348551, Japan Hiroshima Univ Kasumi 1-2-3 Hiroshima Japan 7348551 8551, Japan
Citazione:
E. Kinoshita et al., "Novel wasp toxin discriminates between neuronal and cardiac sodium channels", MOLEC PHARM, 59(6), 2001, pp. 1457-1463

Abstract

Pompilidotoxins (PMTXs), derived from the venom of solitary wasp has been known to facilitate synaptic transmission in the lobster neuromuscular junction, and a recent further study from rat trigeminal neurons revealed that the toxin slows Na+ channel inactivation without modifying activation process. Here we report that beta -PMTX modifies rat brain type II Na+ channel alpha -subunit (rBII) expressed in human embryonic kidney cells but fails toact on the rat heart alpha -subunit (rH1) at similar concentrations. We constructed a series of chimeric mutants of rBII and rH1 Na+ channels and compared modification of the steady-state Na+ currents by beta -PMTX. We foundthat a difference in a single amino acid between Glu-1616 in rBII and Gln-1615 in rH1 at the extracellular loop of D4S3-S4 is crucial for the action of beta -PMTX. PMTXs, which are small peptides with 13 amino acids, would be a potential tool for exploring a new functional moiety of Na+ channels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 00:15:29