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Titolo:
Localization of the ribosomal protection protein Tet(O) on the ribosome and the mechanism of tetracycline resistance
Autore:
Spahn, CMT; Blaha, G; Agrawal, RK; Penczek, P; Grassucci, RA; Trieber, CA; Connell, SR; Taylor, DE; Nierhaus, KH; Frank, J;
Indirizzi:
Hlth Res Inc, Howard Hughes Med Inst, Albany, NY 12201 USA Hlth Res Inc Albany NY USA 12201 rd Hughes Med Inst, Albany, NY 12201 USA Wadsworth Ctr, Albany, NY 12201 USA Wadsworth Ctr Albany NY USA 12201Wadsworth Ctr, Albany, NY 12201 USA SUNY Albany, Dept Biomed Sci, Albany, NY 12222 USA SUNY Albany Albany NY USA 12222 ny, Dept Biomed Sci, Albany, NY 12222 USA Max Planck Inst Mol Genet, AG Ribosomen, D-14195 Berlin, Germany Max Planck Inst Mol Genet Berlin Germany D-14195 D-14195 Berlin, Germany Univ Alberta, Dept Med Microbiol & Immunol, Edmonton, AB T6G 2H7, Canada Univ Alberta Edmonton AB Canada T6G 2H7 nol, Edmonton, AB T6G 2H7, Canada
Titolo Testata:
MOLECULAR CELL
fascicolo: 5, volume: 7, anno: 2001,
pagine: 1037 - 1045
SICI:
1097-2765(200105)7:5<1037:LOTRPP>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLI 70S RIBOSOME; ELONGATION-FACTOR-G; ESCHERICHIA-COLI; ANGSTROM-RESOLUTION; CRYOELECTRON MICROSCOPY; THERMUS-THERMOPHILUS; CRYSTAL-STRUCTURE; SUBUNIT; RNA; VISUALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Frank, J Hlth Res Inc, Howard Hughes Med Inst, Empire State Plaza, Albany,NY 12201USA Hlth Res Inc Empire State Plaza Albany NY USA 12201 , NY 12201USA
Citazione:
C.M.T. Spahn et al., "Localization of the ribosomal protection protein Tet(O) on the ribosome and the mechanism of tetracycline resistance", MOL CELL, 7(5), 2001, pp. 1037-1045

Abstract

Tet(O) belongs to a class of ribosomal protection proteins that mediate tetracycline resistance. It is a G protein that shows significant sequence similarity to elongation factor EF-G. Here we present a cryo-electron microscopic reconstruction, at 16 Angstrom resolution, of its complex with the E. coli 70S ribosome. Tet(O) was bound in the presence of a noncleavable GTP analog to programmed ribosomal complexes carrying fMet-tRNA in the P site. Tet(O) is directly visible as a mass close to the A-site region, similar in shape and binding position to EF-G. However, there are important differences. One of them is the different location of the tip of domain IV, which in the Tet(O) case, does not overlap with the ribosomal A site but is directlyadjacent to the primary tetracycline binding site. Our findings give insights into the mechanism of tetracycline resistance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 15:01:20