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Titolo:
Neuronal targeting of cardiotrophin-1 by coupling with tetanus toxin C fragment
Autore:
Bordet, T; Castelnau-Ptakhine, L; Fauchereau, F; Friocourt, G; Kahn, A; Haase, G;
Indirizzi:
INSERM, U382, Inst Biol Dev Marseille, F-13288 Marseille, France INSERM Marseille France F-13288 Dev Marseille, F-13288 Marseille, France INSERM, U129, Inst Cochin Genet Mol, F-75014 Paris, France INSERM Paris France F-75014 Inst Cochin Genet Mol, F-75014 Paris, France
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 5, volume: 17, anno: 2001,
pagine: 842 - 854
SICI:
1044-7431(200105)17:5<842:NTOCBC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CILIARY NEUROTROPHIC FACTOR; PROGRESSIVE MOTOR NEURONOPATHY; RETROGRADE AXONAL-TRANSPORT; AMYOTROPHIC-LATERAL-SCLEROSIS; SPINAL MUSCULAR-ATROPHY; CENTRAL-NERVOUS-SYSTEM; ACUTE-PHASE RESPONSE; EMBRYONIC MOTONEURONS; ADENOVIRAL VECTORS; FACTOR PREVENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Haase, G INSERM, U382, Inst Biol Dev Marseille, Campus Luminy,Case 907, F-13288 Marseille, France INSERM Campus Luminy,Case 907 Marseille France F-13288 e, France
Citazione:
T. Bordet et al., "Neuronal targeting of cardiotrophin-1 by coupling with tetanus toxin C fragment", MOL CELL NE, 17(5), 2001, pp. 842-854

Abstract

Cardiotrophin-1 (CT-1) is a potent neurotrophic factor for motoneurons butits clinical use in motor neuron diseases is precluded by side effects on the heart and liver. We explored the possibility of targeting CT-1 to neurons by coupling with the tetanus toxin fragment TTC. Genetic fusion proteinsbetween CT-1 or GFP and TTC were produced in Escherichia coil and assayed in vitro, In contrast to uncoupled CT-1 or GFP, TTC-coupled proteins bound with high affinity to cerebral neurons and spinal cord motoneurons and wererapidly internalized. Glia, hepatocytes, or cardiomyocytes did not show detectable binding or uptake of TTC-coupled proteins. Similar to CT-1, TTC-coupled CT-1 induced IL-6 secretion by KB cells, activated Rag-a gene expression, and promoted motoneuron survival in a dose-dependent manner. In vivo studies will test whether TTC-coupled CT-1 might be targeted to degeneratingspinal cord or brain-stem motoneurons and migrate trans-synaptically to cortical motoneurons, which are also affected in amyotrophic lateral sclerosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:12:34