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Titolo:
Characterisation of Wnt gene expression during the differentiation of murine embryonic stem cells in vitro: role of Wnt3 in enhancing haematopoietic differentiation
Autore:
Lako, M; Lindsay, S; Lincoln, J; Cairns, PM; Armstrong, L; Hole, N;
Indirizzi:
Univ Durham, Dept Biol Sci, Durham DH1 3LE, England Univ Durham Durham England DH1 3LE ept Biol Sci, Durham DH1 3LE, England Univ Newcastle Upon Tyne, Inst Human Genet, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England Univ Newcastle Upon Tyne Newcastle Upon Tyne Tyne & Wear England NE1 7RU
Titolo Testata:
MECHANISMS OF DEVELOPMENT
fascicolo: 1-2, volume: 103, anno: 2001,
pagine: 49 - 59
SICI:
0925-4773(200105)103:1-2<49:COWGED>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; MOUSE EMBRYOS; RETINOIC ACID; FAMILY; HEMATOPOIESIS; TRANSFORMATION; EMBRYOGENESIS; PROLIFERATION; DERIVATION; REGULATORS;
Keywords:
Wnt genes; embryonic stem cells; haematopoiesis; GFP; CFU-A; dimethylsulfoxide; retinoic acid; neutrophils; macrophages; hematopoietic stem cells; brachyury; collagen alpha IV; alpha-fetoprotein; activin beta(B); c-kit; GATA-1; flk-1; globin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Lako, M Univ Durham, Dept Biol Sci, South Rd, Durham DH1 3LE, England UnivDurham South Rd Durham England DH1 3LE rham DH1 3LE, England
Citazione:
M. Lako et al., "Characterisation of Wnt gene expression during the differentiation of murine embryonic stem cells in vitro: role of Wnt3 in enhancing haematopoietic differentiation", MECH DEVEL, 103(1-2), 2001, pp. 49-59

Abstract

The first haematopoietic stem cells in mammalian and non-mammalian vertebrates are derived from mesoderm, therefore genes that are important in mesoderm patterning and formation might also play an essential role in haematopoietic stem cell commitment and differentiation. Several members of the Wnt gene family are expressed in very specific patterns in embryonic mesoderm and have previously been shown to act as haematopoietic growth factors. In order to investigate in detail the role that such secreted proteins play in the biology of early haematopoietic commitment we have used in vitro differentiation of murine embryonal stem (ES) as a model system. Using reverse-transcriptase polymerase chain reaction analysis we identified several candidate Wnt genes whose expression pattern was consistent with a role in generation, maintenance and/or differentiation of early haematopoietic progenitorcells including three genes previously shown to have a role in haematopoiesis (Wnt5a, Wnt2b and Wnr10b). The most interesting candidate was Wnt3. because of its strong and regulated expression during in vitro differentiationof murine ES cells as well as its early embryonic expression in mesoderm. Overexpression of Wnt3 was sufficient to cause a consistent increase in thenumber of embryoid bodies committing to haematopoiesis further strengthening the evidence that this protein can enhance haematopoietic commitment during in vitro differentiation of ES cells. In addition, overexpression of Wnt3 caused a marked upregulation of Brachyury expression, thus providing some evidence that Brachyury may be one of the target genes for the Wnt3 signalling pathway. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 23:26:38