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Titolo:
Norsalsolinol uptake into secretory vesicles via vesicular monoamine transporter and its secretion by membrane depolarization or purinoceptor stimulation in PC12 cells
Autore:
Maruyama, Y; Suzuki, Y; Kazusaka, A; Fujita, S;
Indirizzi:
Hokkaido Univ, Grad Sch Vet Med, Lab Toxicol, Dept Environm Vet Sci, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 5, volume: 63, anno: 2001,
pagine: 493 - 497
SICI:
0916-7250(200105)63:5<493:NUISVV>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL CHROMAFFIN CELLS; TYROSINE-HYDROXYLASE; SALSOLINOL; DOPAMINE; GRANULES; STORAGE; 1-METHYL-4-PHENYLPYRIDINIUM; NEUROTOXIN; RELEASE; BRAIN;
Keywords:
exocytosis; norsalsolinol; PC12 cell; vesicular monoamine transporter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Fujita, S Hokkaido Univ, Grad Sch Vet Med, Lab Toxicol, Dept Environm Vet Sci, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan0600818 ido 0600818, Japan
Citazione:
Y. Maruyama et al., "Norsalsolinol uptake into secretory vesicles via vesicular monoamine transporter and its secretion by membrane depolarization or purinoceptor stimulation in PC12 cells", J VET MED S, 63(5), 2001, pp. 493-497

Abstract

The intracellular dynamics of norsalsolinol, a neurotoxin candidate causing parkinsonism-like symptoms, in PC12 cells was studied. We found that dopamine and norsalsolinol are co-localized to secretory granule layer by sucrose density gradient in norsalsolinol-treated PC12 cells. The norsalsolinol was actively taken up into isolated secretory vesicle fraction from PC12 cells with a Km Value of 41.5 +/- 6.8 muM. The uptake of 10 muM of norsalsolinol was sensitive to reserpine (1 muM), an inhibitor of vesicular dopamine transporter, and dopamine, an endogenous substrate, but insensitive to GBR-12909, an inhibitor of dopamine transporter on plasma membrane. In norsalsolinol-treated PC12 cells, exposure to high K+ or ATP resulted in simultaneous release of norsalsolinol and dopamine. Time course of a release of dopamine and that of norsalsolinol evoked by 50 mM KCI or 100 muM ATP corresponded to each other. These releases were dependent on the concentrations of secretagogues. These data suggest that norsalsolinol is taken up with dopamine into secretory vesicle via vesicular catecholamine transporter.

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Documento generato il 26/01/20 alle ore 17:09:50