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Titolo:
TNF-238A promoter polymorphism contributes to susceptibility to ankylosingspondylitis in HLA-B27 negative patients
Autore:
Gonzalez, S; Torre-Alonso, JC; Martinez-Borra, J; Sanchez, JAF; Lopez-Vazquez, A; Perez, AR; Lopez-Larrea, C;
Indirizzi:
Hosp Cent Asturias, Dept Immunol, E-33006 Oviedo, Spain Hosp Cent Asturias Oviedo Spain E-33006 t Immunol, E-33006 Oviedo, Spain
Titolo Testata:
JOURNAL OF RHEUMATOLOGY
fascicolo: 6, volume: 28, anno: 2001,
pagine: 1288 - 1293
SICI:
0315-162X(200106)28:6<1288:TPPCTS>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; TRIPLET REPEAT POLYMORPHISM; PRIMERS PCR-SSP; TNF-ALPHA; POSITIVE INDIVIDUALS; PSORIATIC-ARTHRITIS; MONONUCLEAR-CELLS; ASSOCIATION; REGION; HLA;
Keywords:
HLA-B27 negative; ankylosing spondylitis; tumor necrosis factor-alpha; spondyloarthropathy; polymorphism; MICA gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Lopez-Larrea, C Hosp Cent Asturias, Dept Immunol, E-33006 Oviedo, Spain Hosp Cent Asturias Oviedo Spain E-33006 06 Oviedo, Spain
Citazione:
S. Gonzalez et al., "TNF-238A promoter polymorphism contributes to susceptibility to ankylosingspondylitis in HLA-B27 negative patients", J RHEUMATOL, 28(6), 2001, pp. 1288-1293

Abstract

Objective. To investigate the relative contribution of MHC loci in their susceptibility to primary ankylosing spondylitis (AS) in HLA-B27 negative patients and to compare the clinical features and genetic factors with those of HLA-B27 positive AS. Methods. DNA from patients with B27 negative primary AS (n = 28), B27 positive primary AS (n = 77), and matched healthy controls (B27-, n = 100; B27+, n = 70) were analyzed to investigate whether HLA genes determine the disease susceptibility, or whether other closely linked loci might play a role in disease development. HLA typing was carried out by serology and PCR/SSP (HLA-B, -DR), MICA-TM polymorphism in the transmembrane region by radioactive PCR, and tumor necrosis factor-alpha (TNF-alpha) promoter polymorphism at positions -238 and -308 by PCR-RFLP. Results. Subtle clinical differences were found for primary AS, the B27 negative patients being less frequently complicated by acute anterior uveitisand more associated with peripheral arthritis than B27 positive. Differences were found in the distribution of TNF-alpha -238 genotypes among patients with primary AS (B27- vs B27+). The TNF-alpha -238(A) polymorphism was present in 50% of the B27 negative patients carrying the -238 G/A and A/A genotypes and was significantly increased compared with B27 positive AS (odds ratio 4.3) and with the B27 negative control group (OR 5.9), The TNF-alpha genotypes were equally prevalent in B27 positive AS and healthy matched B27positive controls. No significant HLA and MICA typing differences were found between the populations under study. Conclusion. Our results indicate that the polymorphism variation in the TNF-alpha promoter -238.2(A) influences disease susceptibility in B27 negative primary AS but had no effect in our B27 positive AS population.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 00:48:24