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Titolo:
Alpha-motoneurons of the injured cervical spinal cord of the adult rat canreinnervate the biceps brachii muscle by regenerating axons through peripheral nerve bridges: Combined ultrastructural and retrograde axonal tracing study
Autore:
Rhrich-Haddout, F; Kassar-Duchossoy, L; Bauchet, L; Destombes, J; Thiesson, D; Butler-Browne, G; Lyoussi, B; Baillet-Derbin, C; Horvat, JC;
Indirizzi:
Univ Paris 05, Neurobiol Lab, F-75270 Paris 06, France Univ Paris 05 Paris France 06 5, Neurobiol Lab, F-75270 Paris 06, France Univ Paris 05, CNRS, URA 2115, F-75270 Paris, France Univ Paris 05 ParisFrance F-75270 CNRS, URA 2115, F-75270 Paris, France Fac Sci, Physiol Anim Lab, Fes, Morocco Fac Sci Fes MoroccoFac Sci, Physiol Anim Lab, Fes, Morocco
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 5, volume: 64, anno: 2001,
pagine: 476 - 486
SICI:
0360-4012(20010601)64:5<476:AOTICS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-MOTONEURONS; MOTOR NUCLEI; C SYNAPSES; CAT; NEURONS; IMPLANTATION; RECOVERY; PERONEAL; ATROPHY; PLEXUS;
Keywords:
spinal cord injury; brachial plexus avulsion; peripheral nerve grafting; motoneuron ultrastructure; synaptic covering; muscle reinnervation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Horvat, JC Univ Paris 05, Neurobiol Lab, 45 Rue St Peres, F-75270 Paris 06, France Univ Paris 05 45 Rue St Peres Paris France 06 Paris 06, France
Citazione:
F. Rhrich-Haddout et al., "Alpha-motoneurons of the injured cervical spinal cord of the adult rat canreinnervate the biceps brachii muscle by regenerating axons through peripheral nerve bridges: Combined ultrastructural and retrograde axonal tracing study", J NEUROSC R, 64(5), 2001, pp. 476-486

Abstract

Following our previous studies related to brachial plexus injury and repair, the present experimentation was designed to examine the ultrastructural features of those motoneurons of the locally injured cervical spinal cord of adult rats that were seen to regenerate into peripheral nerve (PN) bridges and to reinnervate nearby skeletal muscles. Here, the peripheral connection of the PN bridge was made with the biceps brachii (BB) muscle. Three months postsurgery, the spinal motoneurons labelled by retrograde axonal transport of horseradish peroxidase (HRP), after its injection into the BE, wereselected on thick sections, using light microscopy, for the presence of dark amorphous granules of the HRP reaction product. Serial ultrathin sections were then made from the selected material. For the 10 labelled neurons studied, we examined the synaptic boutons present on the membrane of the neuronal soma. For five of them, we could observe three of the six types of synaptic boutons described for the alpha-motoneurons of the cat (S-type with spherical vesicles, F-types with flattened vesicles, and C-type with subsynaptic cistern). The largest boutons (type C) are specific to alpha-motoneurons. In comparison to normal material, we noticed a decrease in the number of boutons and an increase in the number of glial processes. After a transient phase of trophic changes, the reinnervated BE muscles showed a return oftheir fibers to nearly normal diameters as well as evidence of fiber type grouping. Simultaneous staining with silver and cholinesterase also revealed the presence of new motor endplates frequently contacted by several motoneurons. The present study indicates that, after a local spinal injury, typical alpha-motoneurons can reinnervate a skeletal muscle by regenerating axons into the permissive microenvironment provided by a PN graft. These data offer prospects for clinical reconstruction of the brachial plexus after avulsion of one or several nerve roots. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 25/09/20 alle ore 09:11:54