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Titolo:
Plasma membrane calcium ATPase plays a role in reducing Ca2+-mediated cytotoxicity in PC12 cells
Autore:
Garcia, ML; Usachev, YM; Thayer, SA; Strehler, EE; Windebank, AJ;
Indirizzi:
Mayo Clin, Program Mol Neurosci, Rochester, MN 55905 USA Mayo Clin Rochester MN USA 55905 am Mol Neurosci, Rochester, MN 55905 USA Mayo Clin & Mayo Grad Sch Med, Dept Biochem & Mol Biol, Rochester, MN 55901 USA Mayo Clin & Mayo Grad Sch Med Rochester MN USA 55901 hester, MN 55901 USA Mayo Med Sch, Dept Neurol, Rochester, MN USA Mayo Med Sch Rochester MN USA yo Med Sch, Dept Neurol, Rochester, MN USA Univ Minnesota, Dept Pharmacol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 rmacol, Minneapolis, MN 55455 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 6, volume: 64, anno: 2001,
pagine: 661 - 669
SICI:
0360-4012(20010615)64:6<661:PMCAPA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT HIPPOCAMPAL SLICE; NA+/K+-PUMP ATPASE; CA2+ PUMP; CA-2+-TRANSPORTING ATPASE; FUNCTIONAL DOMAINS; MOLECULAR-CLONING; TRANSPORT ATPASES; MESSENGER-RNA; CA2+-ATPASE; ISOFORM;
Keywords:
apoptosis; calcium ATPase; cell death; PC12 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Windebank, AJ Mayo Clin, Program Mol Neurosci, 1501 Guggenheim Bldg,200 1st St SW, Rochester, MN 55905 USA Mayo Clin 1501 Guggenheim Bldg,200 1st St SW Rochester MN USA 55905
Citazione:
M.L. Garcia et al., "Plasma membrane calcium ATPase plays a role in reducing Ca2+-mediated cytotoxicity in PC12 cells", J NEUROSC R, 64(6), 2001, pp. 661-669

Abstract

In many cell types, cell death induced by a variety of insults is accompanied by an increase in intracellular calcium, The Ca2+ homeostatic mechanisms affected by such insults, however, have not been fully determined. Recentevidence indicates that kainic acid-induced seizures alter plasma membranecalcium ATPase mRNA expression within vulnerable hippocampal cell populations before the onset of cell death. We examined the effects of altering plasma membrane calcium ATPase expression on cell vulnerability in rat pheochromocytoma 12 cells. Pheochromocytoma 12 cells ave vulnerable to Ca2+ overload induced by the Ca2+ ionophore A23187. Reverse transcriptase-PCR and Western blot data indicated that plasma membrane calcium ATPase isoform 4b constitutes a major calcium pump isoform in the pheochromocytoma 12 cells. Therefore, permanently transfected pheochromocytoma 12-derived cell lines were established that either over-expressed plasma membrane calcium ATPase isoform 4b, or suppressed the expression of the endogenous plasma membrane calcium ATPase isoform 4, Over-expressing clones were less vulnerable to Ca2+-mediated cell death induced by A23187 whereas "antisense" clones were considerably more susceptible. These data indicate that regulation of plasma membrane calcium ATPase expression may be critical to cellular survival when cells are exposed to pathological increases in intracellular calcium. (C) 2001Wiley-Liss, Inc.

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Documento generato il 25/01/20 alle ore 16:11:52