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Titolo:
Genetic variability of respiratory syncytial virus subgroup a strain in 15successive epidemics in one city
Autore:
Seki, K; Tsutsumi, H; Ohsaki, M; Kamasaki, H; Chiba, S;
Indirizzi:
Sapporo Med Univ, Sch Med, Dept Pediat, Sapporo, Hokkaido, Japan Sapporo Med Univ Sapporo Hokkaido Japan Pediat, Sapporo, Hokkaido, Japan
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 3, volume: 64, anno: 2001,
pagine: 374 - 380
SICI:
0146-6615(200107)64:3<374:GVORSV>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MISMATCH CLEAVAGE METHOD; AMINO-ACID CHANGES; G-GLYCOPROTEIN; MOLECULAR EPIDEMIOLOGY; MONOCLONAL-ANTIBODIES; B STRAINS; EVOLUTIONARY PATTERN; INFLUENZA-A; G-PROTEIN; HETEROGENEITY;
Keywords:
respiratory syncytial virus; nucleoprotein; attachment protein; polymerase chain reaction; restriction fragment; length polymorphism; neighbor joining method;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Tsutsumi, H Hokkaido Univ, Sch Med, Dept Pediat, Chuoku S-1,W-16, Sapporo,Hokkaido 0608543, Japan Hokkaido Univ Chuoku S-1,W-16 Sapporo Hokkaido Japan 0608543 n
Citazione:
K. Seki et al., "Genetic variability of respiratory syncytial virus subgroup a strain in 15successive epidemics in one city", J MED VIROL, 64(3), 2001, pp. 374-380

Abstract

The genetic variability of 125 respiratory syncytial virus (RSV) subgroup A isolates over 15 successive epidemics from 1980 to 1995 in an urban population of Japan was determined. Allocation of isolates into lineages was archived by reverse transcriptase-polymerase chain reaction amplification of selected regions of the nucleoprotein (NP) and attachment (G) protein gene followed by restriction fragment length polymorphism (RFLP) analysis. Three and seven distinct restriction patterns of the NP and G gene were observed,respectively. When the NP and G gene RFLP analyses were combined, ten different genetic lineages were identified in the 125 isolates. The strains with the same genotype were isolated in each epidemic and the dominant lineages were replaced by others after every one to three consecutive epidemics. Nucleotide and amino acid sequencing of the variable region of G gene of these predominant isolates revealed differences of 5-28% between strains. There was, however, no apparent accumulation of diversity with age to indicate progressive changes. The dominant strains were often closely related to those isolated in other parts of the world at a similar time. These observations suggest that dominant RSV strains are replaced frequently by others thathave been co-circulating or have recently entered the community from a worldwide reservoir. The change of dominant strains may be influenced by the buildup of immunological resistance in the community to successive epidemicsof the same strain. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:52:18