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Titolo:
Molecular epidemiology of Escherichia coli isolated from young South African children with diarrhoeal diseases
Autore:
Galane, PM; Le Roux, M;
Indirizzi:
Med Univ So Africa, Dept Med Microbiol, MRC, Medunsa Diarrhoeal Pathogens Unit, ZA-0204 Medunsa, South Africa Med Univ So Africa Medunsa South Africa ZA-0204 04 Medunsa, South Africa
Titolo Testata:
JOURNAL OF HEALTH POPULATION AND NUTRITION
fascicolo: 1, volume: 19, anno: 2001,
pagine: 31 - 38
SICI:
1606-0997(200103)19:1<31:MEOECI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
BUNDLE-FORMING PILUS; AGGREGATIVE ADHERENCE; INFANTILE DIARRHEA; SEROTYPE O157-H7; RURAL CHILDREN; STRAINS; PREVALENCE; VIRULENCE; ENTEROPATHOGENS; ENTEROADHERENT;
Keywords:
Escherichia coli; diarrhoea; serotyping; polymerase chain reaction; tissue culture; South Africa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Le Roux, M Med Univ So Africa, Dept Med Microbiol, MRC, Medunsa DiarrhoealPathogens Unit, POB 204, ZA-0204 Medunsa, South Africa Med Univ So Africa POB 204 Medunsa South Africa ZA-0204 Africa
Citazione:
P.M. Galane e M. Le Roux, "Molecular epidemiology of Escherichia coli isolated from young South African children with diarrhoeal diseases", J HEALTH PO, 19(1), 2001, pp. 31-38

Abstract

Molecular techniques were used for studying the epidemiology of diarrhoealinfections due to Escherichia coli in the Gauteng region in South Africa. In total, 151 E, coli strains isolated from stools of patients with diarrhoea and 30 strains isolated from stools of healthy individuals were collected between March 1996 and May 1997, The E, coli isolates were characterized by serotyping, antimicrobial susceptibility testing, and adherence patterns, Polymerase chain reaction (PCR) was performed to determine the presence of the genes-encoding virulence factors. PCR showed that 59 (32.6 %) of the E. coil isolates carried eaeA genes, 6 (3.3 %) possessed bfpA genes, 4 (2.2%) CNF1, and 2 (1.1%) carried labile toxin and Stx2 genes. The eae genes were more prevalent in strains isolated from patients than in those from the control group (p <0.001). Forty-eight (26.5 %) strains belonged to enteropathogenic E, coil (EPEC) O serogroups and 14 (7.7%) to Shiga toxin-producingE. coil (STEC) O157 serotype, A high percentage (28.2%) of atypical EPEC strains possessing the eaeA but not the bfpA genes was isolated. Most isolates were susceptible to commonly-used antimicrobial agents. The adherence ofthe E. coli strains to HeLa cells was identified more in patients (69.4%) than in the control group (60%) and was more dominant in infants than in adults. PCR and tissue culture assays were shown to be useful techniques for the epidemiological study of E, coil where this organism is a major cause of diarrhoea.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:31:36