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Titolo:
Human endothelial cells grow poorly on vitronectin: Role of PAI-1
Autore:
Underwood, PA; Bean, PA; Cubeddu, L;
Indirizzi:
CSIRO, Mol Sci, N Ryde, NSW 1670, Australia CSIRO N Ryde NSW Australia 1670 IRO, Mol Sci, N Ryde, NSW 1670, Australia Macquarie Univ, Dept Chem, Sydney, NSW 2109, Australia Macquarie Univ Sydney NSW Australia 2109 hem, Sydney, NSW 2109, Australia
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 82, anno: 2001,
pagine: 98 - 109
SICI:
0730-2312(2001)82:1<98:HECGPO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR INHIBITOR-1; MONOCLONAL-ANTIBODIES; UROKINASE RECEPTOR; BIOLOGICAL-ACTIVITIES; EXTRACELLULAR-MATRIX; ADHESION RECEPTOR; HT-1080 CELLS; MIGRATION; IDENTIFICATION; PROLIFERATION;
Keywords:
endothelial; vitronectin; PAI-1; uPA; uPAR; cell adhesion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Underwood, PA CSIRO, Mol Sci, 2 Richardson Pl,Riverside Corp Pk,Delhi Rd,POB 18, N Ryde,NSW 1670, Australia CSIRO 2 Richardson Pl,Riverside Corp Pk,Delhi Rd,POB 18 N Ryde NSW Australia 1670
Citazione:
P.A. Underwood et al., "Human endothelial cells grow poorly on vitronectin: Role of PAI-1", J CELL BIOC, 82(1), 2001, pp. 98-109

Abstract

The cell adhesive protein vitronectin is a common component of interstitial extracellular matrix and circulates in plasma. it competes effectively with other plasma proteins to adsorb to certain biomaterial surfaces, and is likely to represent an important cell adhesion mediator on the luminal surface of vascular grafts. It is also found associated with certain vascular pathologies. We have shown previously that human endothelial cells grow poorly on a vitronectin surface compared with other extracellular matrix molecules. In this paper we show that endothelial cells seeded on vitronectin andfibronectin produced substantially different profiles of extracellular matrix molecules. The most outstanding difference was in the amount of matrix-localised plasminogen activator-inhibitor-1 which was high on vitronectin and negligible on fibronectin. This was correlated with a small but significant inhibition of cell adhesion to vitronectin compared with fibronectin, and very significant interference with dissociation of cell: extracellular matrix contacts, resulting either from direct inhibition of the proteolytic activity of urokinase, or from interference with urokinase-receptor signaling and consequent focal adhesion turnover. Such interference would inhibit cell proliferation by disabling the cells from loosening their matrix contacts in order to proceed through mitosis. This would seriously compromise endothelial recovery in cases of damage to the vascular wall and placement ofstents or grafts, where the presence of surface-adsorbed vitronectin is likely to modulate the tissue response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 18:56:10