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Titolo:
Human T cell responses to endogenously presented HLA-A*0201 restricted peptides of simian virus 40 large T antigen
Autore:
Velders, MP; Macedo, MF; Provenzano, M; Elmishad, AG; Holzhutter, HG; Carbone, M; Kast, WM;
Indirizzi:
Loyola Univ, Cardinal Bernardin Canc Ctr, Canc Immunol Program, Maywood, IL 60153 USA Loyola Univ Maywood IL USA 60153 c Immunol Program, Maywood, IL 60153 USA Humboldt Univ, Fac Med, Inst Biochem, D-1086 Berlin, Germany Humboldt Univ Berlin Germany D-1086 Inst Biochem, D-1086 Berlin, Germany
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 82, anno: 2001,
pagine: 155 - 162
SICI:
0730-2312(2001)82:1<155:HTCRTE>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PLEURAL MESOTHELIOMA; DENDRITIC CELLS; TUMOR-ANTIGEN; IN-VIVO; SIMIAN-VIRUS-40; SV40; TRANSFORMATION; PROTEASOME; GENERATION; INDUCTION;
Keywords:
SV40; CTL; peptide; HLA-A*0201; mesothelioma; immunotherapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Kast, WM Loyola Univ, Cardinal Bernardin Canc Ctr, Canc Immunol Program, 2160 S 1stAve, Maywood, IL 60153 USA Loyola Univ 2160 S 1st Ave Maywood IL USA 60153 ood, IL 60153 USA
Citazione:
M.P. Velders et al., "Human T cell responses to endogenously presented HLA-A*0201 restricted peptides of simian virus 40 large T antigen", J CELL BIOC, 82(1), 2001, pp. 155-162

Abstract

Presence of the simian virus 40 (SV40) has recently been demonstrated in arelatively high percentage of hu man mesotheliomas and it is associated with the development of these malignancies in pleural cavities. Therefore, wehave initiated a study to identify candidate peptides presented by the human HLA-A*0201 molecule for vaccination approaches against SV40 and monitoring of SV40 directed human immune responses. initial screening of SV40 largeT (Tag) domains required for transformation of cells for HLA-A*0201 binding motifs revealed ten possible binding peptides. Screening of these candidate peptides showed that seven of the ten peptides could bind and stabilize HLAA*0201 molecules. In an in vitro immunization assay the two peptides with the highest binding affinity for HLA-A*0201, Tag aa 396-405 and aa 577-585, were tested for their ability to induce peptide specific cytotoxic T cells in two healthy donors. One donor developed cytotoxic T cells against Tagaa 396-405 and in T cell cultures of both donors Tag aa 577-585 specific Tcells were initiated. The T cells against Tag aa 577-585 not only recognized and killed peptide pulsed cells, but, most importantly, SV40 transformedhuman mesothelial cells. This is the first demonstration of the induction of SV40 specific human cytotoxic T lymphocytes that recognize endogenously processed peptides from SV40. This peptide identification study opens the possibility to investigate immune responses against SV40 in mesothelioma patients and in individuals exposed to SV40.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:06:31