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Titolo:
Membrane-type 1 matrix metalloproteinase cleaves CD44 and promotes cell migration
Autore:
Kajita, M; Itoh, Y; Chiba, T; Mori, H; Okada, A; Kinoh, H; Seiki, M;
Indirizzi:
Univ Tokyo, Dept Canc Cell Res, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Med Sci, Minato Ku, Tokyo 1088639, Japan
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 5, volume: 153, anno: 2001,
pagine: 893 - 904
SICI:
0021-9525(20010528)153:5<893:M1MMCC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR-MATRIX; SOLUBLE CD44; RHEUMATOID-ARTHRITIS; L-SELECTIN; INTERGLOBULAR DOMAIN; COLORECTAL-CANCER; CARCINOMA-CELLS; MELANOMA-CELLS; SERUM LEVELS; IN-VITRO;
Keywords:
MT-MMP; metalloproteinase; motility; CD44; invasion and metastasis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Seiki, M Univ Tokyo, Dept Canc Cell Res, Inst Med Sci, Minato Ku, 4-6-1 Shirokane Dai, Tokyo 1088639, Japan Univ Tokyo 4-6-1 Shirokane Dai Tokyo Japan 1088639 088639, Japan
Citazione:
M. Kajita et al., "Membrane-type 1 matrix metalloproteinase cleaves CD44 and promotes cell migration", J CELL BIOL, 153(5), 2001, pp. 893-904

Abstract

Migratory cells including invasive tumor cells frequently express CD44, a major receptor for hyaluronan and membrane-type 1 matrix metalloproteinase (MT1-MMP) that degrades extracellular matrix at the pericellular region. Inthis study, we demonstrate that MT1-MMP acts as a processing enzyme for CD44H, re leasing it into the medium as a soluble 70-kD fragment. Furthermore, this processing event stimulates cell motility, however, expression of either CD44H or MT1-MMP alone did not stimulate cell motility. Coexpression of MT1-MMP and mutant CD44H lacking the MT1-MMP-processing site did not result in shedding and did not promote cell migration, suggesting that the processing of CD44H by MT1-MMP is critical in the migratory stimulation. Moreover, expression of the mutant CD44H inhibited the cell migration promoted byCD44H and MT1-MMP in a dominant-negative manner. The pancreatic tumor cellline, MIA PaCa-2, was found to shed the 70-kD CD44H fragment in a MT1-MMP-dependent manner. Expression of the mutant CD44H in the cells as well as MMP inhibitor treatment effectively inhibited the migration, suggesting that MIA PaCa-2 cells indeed use the CD44H and MT1-MMP as migratory devices. These findings revealed a novel interaction of the two molecules that have each been implicated in tumor cell migration and invasion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 22:37:35