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Titolo:
Physiological and genomic consequences of intermittent hypoxia - Selected contribution: Osteocytes upregulate HIF-1 alpha in response to acute disuseand oxygen deprivation
Autore:
Gross, TS; Akeno, N; Clemens, TL; Komarova, S; Srinivasan, S; Weimer, DA; Mayorov, S;
Indirizzi:
Univ Cincinnati, Dept Orthopaed, Cincinnati, OH 45267 USA Univ CincinnatiCincinnati OH USA 45267 thopaed, Cincinnati, OH 45267 USA Univ Cincinnati, Dept Med, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 ept Med, Cincinnati, OH 45267 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 6, volume: 90, anno: 2001,
pagine: 2514 - 2519
SICI:
8750-7587(200106)90:6<2514:PAGCOI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; OSTEOCLASTIC BONE-RESORPTION; COLONY-STIMULATING FACTOR; INDUCIBLE FACTOR 1-ALPHA; GAP-JUNCTIONS; UP-REGULATION; CELL-LINE; STABILIZATION; EXPRESSION; MECHANISMS;
Keywords:
hypoxia; bone resorption; vascular endothelial growth factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Gross, TS Univ Washington, Dept Orthopaed & Sports Med, Box 359798,325 9thAve, Seattle, WA 98104 USA Univ Washington Box 359798,325 9th Ave Seattle WA USA 98104 USA
Citazione:
T.S. Gross et al., "Physiological and genomic consequences of intermittent hypoxia - Selected contribution: Osteocytes upregulate HIF-1 alpha in response to acute disuseand oxygen deprivation", J APP PHYSL, 90(6), 2001, pp. 2514-2519

Abstract

Loss of mechanical loading, or disuse, rapidly precipitates locally mediated bone resorption. However, the pathway by which this process is initiatedand mediated is poorly understood. In this study, we used a complementary in vivo and in vitro approach to determine whether disuse-induced osteocytehypoxia resulted in upregulation of the hypoxia-dependent transcription factor HIF-1 alpha. We found that acute disuse (1-5 days) resulted in a significant increase in the percentage of osteocytes staining positive for HIF-1alpha vs. normal bone (30.9 +/- 6.1 vs. 14.1 +/- 3.8%) and that this response was uniform around the cortex. In addition, we found that acute oxygen deprivation (4-12 h of 2% O-2) resulted in a 2.1- to 3.7-fold upregulation of HIF-1 alpha protein expression in MLO-Y4 osteocyte-like cells compared with cells cultured in parallel under normal oxygen conditions. Given known HIF-1 alpha targets genes, we suggest that osteocyte hypoxia and subsequentupregulation of hypoxia-dependent pathways may serve to initiate and mediate disuse-induced bone resorption.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 21:37:08