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Titolo:
Simple freezing apparatus for resolving rapid metabolic events associated with smooth muscle activation
Autore:
Maass-Moreno, R; Burdyga, T; Mitchell, RW; Seow, CY; Ragozzino, J; Ford, LE;
Indirizzi:
Indiana Univ, Sch Med, Krannert Inst Cardiol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ardiol, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Med, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 pt Med, Indianapolis, IN 46202 USA
Titolo Testata:
JOURNAL OF APPLIED PHYSIOLOGY
fascicolo: 6, volume: 90, anno: 2001,
pagine: 2453 - 2459
SICI:
8750-7587(200106)90:6<2453:SFAFRR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOSIN;
Keywords:
tissue freezing; myosin light chain phosphorylation; myosin light chain kinase; arrest of metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Ford, LE Indiana Univ, Sch Med, Krannert Inst Cardiol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ndianapolis, IN 46202 USA
Citazione:
R. Maass-Moreno et al., "Simple freezing apparatus for resolving rapid metabolic events associated with smooth muscle activation", J APP PHYSL, 90(6), 2001, pp. 2453-2459

Abstract

A method is described for freezing thin strips of smooth muscle by replacing physiological saline in the muscle chamber with cold organic solvent in <100 ms. Calculations suggest that, with a perfectly stirred boundary at the tissue surface, freezing could occur within <similar to>15 ms at the center of a 200-mum-thick piece of tissue by use of acetone coolant at -78.5 degreesC and in approximately half the time with either isopentane at its freezing point (-160 degreesC) or aluminum chilled with liquid nitrogen. Myosin light chain phosphorylation in muscles frozen with cold acetone began to rise similar to 200 ms earlier than force and increased at a much more rapid rate. The difference in onsets of the two processes reflects the delay inarresting phosphorylation plus two lags associated with force generation, attachment of phosphorylated bridges followed by force generating movementsof the attached bridges. The much more rapid rise of phosphorylation, onceit began, suggests that most of this delay is due to physiological lags and not to slow arrest of metabolism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:09:05