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Titolo:
Characterization of four new monoclonal antibodies that recognize mouse natural killer activation receptors
Autore:
Kung, SKP; Su, RC; Shannon, J; Miller, RG;
Indirizzi:
Univ Toronto, Ontario Canc Inst, Dept Med Biophys, Toronto, ON M5G 2M9, Canada Univ Toronto Toronto ON Canada M5G 2M9 ophys, Toronto, ON M5G 2M9, Canada
Titolo Testata:
HYBRIDOMA
fascicolo: 2, volume: 20, anno: 2001,
pagine: 91 - 101
SICI:
0272-457X(200104)20:2<91:COFNMA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NK CELLS; NK1.1 ANTIGEN; T-CELLS; MHC; NKR-P1; SPECIFICITY; MOLECULE; CLONING; COMPLEX; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, RG Univ Toronto, Ontario Canc Inst, Dept Med Biophys, 610 Univ Ave, Toronto, ON M5G 2M9, Canada Univ Toronto 610 Univ Ave Toronto ON Canada M5G 2M9 2M9, Canada
Citazione:
S.K.P. Kung et al., "Characterization of four new monoclonal antibodies that recognize mouse natural killer activation receptors", HYBRIDOMA, 20(2), 2001, pp. 91-101

Abstract

With the aim of identifying natural killer (NK) activation receptors, we immunized BALB/c mice with (BALB/cxB6)F1 NK LAK cells and made B-cell hybridomas, These were screened for monoclonal antibody (MAb) reacting with an NKactivation receptor by using an antibody-induced redirected lysis (AIRL) assay against FcR-bearing P815 targets, Four hybridomas, clones 1C10, 1F10, 2D10 and 4G4, were selected for further characterization. Protein G-purified MAbs from these clones activated both resting and IL-2 activated B6 or F1NK cells in the AIRL assay, 1F10 MAb, but not the other three MAbs, could compete for the binding of anti-NK1.1 (PK136) MAb to Fl NK cells. The four MAbs were screened for their ability to bind to or activate NK cells from the mouse strains SJL/J, DBA/2, 129/J, C3H/J, and BALB.K. None showed activity except 1C10, which could bind to and activate SJL/J NK cells. When members of the NKR-P1 family from both B6 mice (A, B, and C genes expressed) andSJL mice (only A and B genes expressed) were expressed in Jurkat cells andtested for their antibody reactivity, PK136 MAb was found to recognize B6 NKR-P1C and SJL/J NKR-P1B; 1C10 MAb was found to recognize NKR-P1-A, -B and-C from B6, but not NKR-P1A or B from SJL/J; and 1F10 MAb was found to react only with B6 NKR-P1C.

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Documento generato il 23/09/20 alle ore 13:08:10