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Titolo:
Cloning, mapping, and characterization of the human sorbin and SH3 domain containing 1 (SORBS1) gene: A protein associated with c-Abl during insulin signaling in the hepatoma cell line Hep3B
Autore:
Lin, WH; Huang, CJ; Liu, MW; Chang, HM; Chen, YJ; Tai, TY; Chuang, LM;
Indirizzi:
Natl Taiwan Univ, Coll Med, Dept Internal Med, Taipei, Taiwan Natl Taiwan Univ Taipei Taiwan l Med, Dept Internal Med, Taipei, Taiwan Natl Taiwan Univ, Coll Med, Grad Inst Clin Med, Taipei, Taiwan Natl TaiwanUniv Taipei Taiwan Med, Grad Inst Clin Med, Taipei, Taiwan Acad Sinica, Inst Biol Chem, Taipei, Taiwan Acad Sinica Taipei TaiwanAcad Sinica, Inst Biol Chem, Taipei, Taiwan Natl Yang Ming Univ, Ctr Gen Educ, Taipei 112, Taiwan Natl Yang Ming UnivTaipei Taiwan 112 , Ctr Gen Educ, Taipei 112, Taiwan
Titolo Testata:
GENOMICS
fascicolo: 1, volume: 74, anno: 2001,
pagine: 12 - 20
SICI:
0888-7543(20010515)74:1<12:CMACOT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE KINASE; TRANSFORMING ACTIVITY; BINDING-PROTEIN; STRESS FIBERS; PIMA-INDIANS; CAP; ACTIVATION; TRANSPORT; INTERACTS; DEFINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Chuang, LM Natl Taiwan Univ Hosp, Dept Internal Med, 7 Chung Shan South Rd, Taipei 100, Taiwan Natl Taiwan Univ Hosp 7 Chung Shan South Rd Taipei Taiwan 100
Citazione:
W.H. Lin et al., "Cloning, mapping, and characterization of the human sorbin and SH3 domain containing 1 (SORBS1) gene: A protein associated with c-Abl during insulin signaling in the hepatoma cell line Hep3B", GENOMICS, 74(1), 2001, pp. 12-20

Abstract

SH3P12/CAP/ponsin, a gene product with a sorbin homology domain and three consecutive SH3 domains in the carboxy-terminus, has been isolated from murine adipocytes and identified as an important adaptor during insulin signaling. Here we describe the cloning, mapping, and expression of the human homologue, termed SORBS1 (sorbin and SH3 domain containing 1). Multiple transcripts of this gene with different mRNA isoforms were observed among different tissues. Here we report 13 alternatively spliced exons, which were ascertained from the full-length cDNA cloned in adipose, liver, and skeletal muscle tissues. Among the major isoforms, the shortest, 2223-bp, open reading frame (ORF) encodes a protein with a predicted molecular weight of 81.5 kDa, while the longest, 3879-bp, ORF encodes a protein of about 142.2 kDa. This gene was mapped to human chromosome 10q23.3q24.1, which is a candidate region for insulin resistance found in Pima Indians. In human hepatoma Hep3B cells, SORBS1 was partly dissociated from the insulin receptor complex and bound to c-Ab1 protein upon insulin stimulation. This interaction with c-Ab1 was through the third SH3 domain and a possible conformational change of SORBS1 induced by insulin. Our data suggest that c-Ab1 oncoprotein via SORBS1 might play a role in the insulin signaling pathway. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 05:14:28