Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Comprehensive genome sequence analysis of a breast cancer amplicon
Autore:
Collins, C; Volik, S; Kowbel, D; Ginzinger, D; Ylstra, B; Cloutier, T; Hawkins, T; Predki, P; Martin, C; Wernick, M; Kuo, WL; Alberts, A; Gray, JW;
Indirizzi:
Univ Calif San Francisco, Ctr Canc, San Francisco, CA 94143 USA Univ CalifSan Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Lawrence Berkeley Lab, Berkeley, CA 94143 USA Lawrence Berkeley Lab Berkeley CA USA 94143 y Lab, Berkeley, CA 94143 USA Joint Genome Inst, Dept Energy, Walnut Creek, CA 94958 USA Joint Genome Inst Walnut Creek CA USA 94958 y, Walnut Creek, CA 94958 USA Novartis Agr Discovery Inst, San Diego, CA 92121 USA Novartis Agr Discovery Inst San Diego CA USA 92121 an Diego, CA 92121 USA Van Andel Inst, Grand Rapids, MI 49503 USA Van Andel Inst Grand Rapids MIUSA 49503 Inst, Grand Rapids, MI 49503 USA
Titolo Testata:
GENOME RESEARCH
fascicolo: 6, volume: 11, anno: 2001,
pagine: 1034 - 1042
SICI:
1088-9051(200106)11:6<1034:CGSAOA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA COPY NUMBER; 20Q13.2 AMPLIFICATION; HUMAN NEOPLASMS; PHYSICAL MAP; 20Q GAIN; IN-SITU; HYBRIDIZATION; REGION; GENES; IMMORTALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Collins, C Univ Calif San Francisco, Ctr Canc, San Francisco, CA 94143 USAUniv Calif San Francisco San Francisco CA USA 94143 94143 USA
Citazione:
C. Collins et al., "Comprehensive genome sequence analysis of a breast cancer amplicon", GENOME RES, 11(6), 2001, pp. 1034-1042

Abstract

Gene amplification occurs in most solid tumors and is associated with poorprognosis. Amplification of 20q13.2 is common to several tumor types including breast cancer. The 1 Mb of sequence spanning the 20q13.2 breast canceramplicon is one of the most exhaustively studied segments of the human genome. These studies have included amplicon mapping by comparative genomic hybridization (CGH], fluorescent in-situ hybridization (FISH), array-CGH, quantitative microsatellite analysis (QUMA), and functional genomic studies. Together these studies revealed a complex amplicon structure suggesting the presence of at least two driver genes in some tumors. One of these, ZNF217,is capable of immortalizing human mammary epithelial cells [HMEC) when overexpressed. In addition, we now report the sequencing of this region in human and mouse, and on quantitative expression studies in tumors. Amplicon localization now is straightforward and the availability of human and mouse genomic sequence facilitates their functional analysis. However, comprehensive annotation of megabase-scale regions requires integration of vast amounts of information. We present a system for integrative analysis and demonstrate its utility on 1.2 Mb of sequence spanning the 20q13.2 breast cancer amplicon and 865 kb of syntenic: murine sequence. We integrate tumor genome copy number measurements with exhaustive genome landscape mapping, showing that amplicon boundaries are associated with maxima in repetitive element density and a region of evolutionary instability. This integration of comprehensive sequence annotation, quantitative expression analysis, and tumor amplicon boundaries provide evidence for an additional driver gene prefoldin 4(PFDN4), coregulated genes, conserved noncoding regions, and associate repetitive elements with regions of genomic instability at this locus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 13:55:47