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Titolo:
Self-splicing of the Tetrahymena group I ribozyme without conserved base-triples
Autore:
Ikawa, Y; Yoshioka, W; Ohki, Y; Shiraishi, H; Inoue, T;
Indirizzi:
Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Grad Sch Biostudies, Kyoto 6068502, Japan
Titolo Testata:
GENES TO CELLS
fascicolo: 5, volume: 6, anno: 2001,
pagine: 411 - 420
SICI:
1356-9597(200105)6:5<411:SOTTGI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRON ACTIVE-SITE; METAL-ION; CATALYTIC ACTIVITY; CRYSTAL-STRUCTURE; 2 DOMAINS; RNA; CORE; FORM; REQUIREMENTS; ELEMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, T Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto UnivKyoto Japan 6068502 Biostudies, Kyoto 6068502, Japan
Citazione:
Y. Ikawa et al., "Self-splicing of the Tetrahymena group I ribozyme without conserved base-triples", GENES CELLS, 6(5), 2001, pp. 411-420

Abstract

Background: Group I introns share a conserved core region consisting of two domains, P8-P3-P7 and P4-P6, joined by four base-triples. We showed previously that the T4 td intron can perform phosphoester transfer reactions at two splice sites in the absence of both P4-P6 and the conserved base-triples, whereas it is barely able to perform the intact splicing reaction due tothe difficulty of conducting the sequential reactions. Results: Based on previous findings, we constructed a bimolecular ribozymelacking a large portion of P4-P6 and the base-triples from the Tetrahymenaintron, on the assumption that the long-range interactions of the peripheral regions in the two RNAs can compensate for the deteriorated core. The bimolecular ribozyme performed the intact splicing reaction. Conclusion: The present analysis indicates that the base-triples are nonessential, but that LA and the distal part of P4 in P4-P6 are important for conducting the splicing reaction. The reconstituted self-splicing ribozyme provides an amenable system for analysing the role(s) of elements in the core region in the self-splicing reaction mechanism.

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Documento generato il 19/01/20 alle ore 01:00:41