Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Novel ERBB4 juxtamembrane splice variants are frequently expressed in childhood medulloblastoma
Autore:
Gilbertson, R; Hernan, R; Pietsch, T; Pinto, L; Scotting, P; Allibone, R; Ellison, D; Perry, R; Pearson, A; Lunec, J;
Indirizzi:
St Jude Childrens Res Hosp, Dept Dev Neurobiol, Memphis, TN 38105 USA St Jude Childrens Res Hosp Memphis TN USA 38105 ol, Memphis, TN 38105 USA Univ Bonn, Med Ctr, Dept Neuropathol, D-5300 Bonn, Germany Univ Bonn Bonn Germany D-5300 tr, Dept Neuropathol, D-5300 Bonn, Germany Univ Newcastle Upon Tyne, Sch Med, Canc Res Unit, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England Univ Newcastle Upon Tyne Newcastle Upon Tyne Tyne & Wear England NE1 7RU Univ Nottingham, Queens Med Ctr, Nottingham NG7 2RD, England Univ Nottingham Nottingham England NG7 2RD , Nottingham NG7 2RD, England Southampton Gen Hosp, Dept Neuropathol, Southampton SO9 4XY, Hants, England Southampton Gen Hosp Southampton Hants England SO9 4XY XY, Hants, England Newcastle Gen Hosp, Dept Neuropathol, Newcastle Upon Tyne NE4 6BE, Tyne & Wear, England Newcastle Gen Hosp Newcastle Upon Tyne Tyne & Wear England NE4 6BE ngland Royal Victoria Infirm, N England Childrens Canc Res Unit, Newcastle Upon Tyne NE1 4LP, Tyne & Wear, England Royal Victoria Infirm Newcastle Upon Tyne Tyne & Wear England NE1 4LP and
Titolo Testata:
GENES CHROMOSOMES & CANCER
fascicolo: 3, volume: 31, anno: 2001,
pagine: 288 - 294
SICI:
1045-2257(200107)31:3<288:NEJSVA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE ENCODES; CELL-LINES; RECEPTOR; TRANSCRIPTS; ISOFORM; GROWTH; HER2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Gilbertson, R St Jude Childrens Res Hosp, Dept Dev Neurobiol, Room 2006G,332 N Lauderdale St, Memphis, TN 38105 USA St Jude Childrens Res Hosp Room 2006G,332 N Lauderdale St Memphis TN USA 38105
Citazione:
R. Gilbertson et al., "Novel ERBB4 juxtamembrane splice variants are frequently expressed in childhood medulloblastoma", GENE CHROM, 31(3), 2001, pp. 288-294

Abstract

We recently reported a significant relationship between tumor cell expression of the ERBB4 receptor, the most recently described member of the epidermal growth factor receptor family, and aggressive tumor phenotype in childhood medullo-blastoma. Two alternative juxtamembrane (JM) isoforms of the ERBB4 receptor have been described. Termed JMa and JMb, these variants possess different receptor processing and ligand-binding characteristics. In the current study, we employed an RT-PCR and sequencing strategy to determine the pattern of ERBB4 IM isoform expression in a targe (n = 78) series of pediatric medulloblastomas. JMa and JMb transcript expression was detected in 53% and 28% of tumor samples, respectively. In addition, two novel ERBB4 JMisoforms, which we have termed JMe and JMd, were isolated from 10% and 36%of tumors, respectively. Sequence analysis revealed the JMc transcript to contain a deletion of the entire JM region. In contrast, JMd includes an extended coding region, retaining both the JMa and JMb sequences. Neither of these novel isoforms was detected in normal human adult cerebellum but expression of JMd was observed in developing fetal cerebellum, suggesting that this later isoform may represent an ERBB4 transcript restricted to primitive neuroectoderm-derived tissue. To confirm that the four ERBB4 JM isoforms arise by alternative RNA splicing, we sequenced the intron-exon junctions of the human ERBB4 gene within the JM region. This demonstrated the four ERBB4 JM variants to be encoded by two short exons containing the JMb and JMa sequences positioned in the order 5 ' to 3 ' and separated by a 121 bp intron. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:44:56