Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP)
Autore:
Dos Santos, NMS; Taverne-Thiele, JJ; Barnes, AC; Ellis, AE; Rombout, JHWM;
Indirizzi:
Univ Porto, Inst Mol & Cell Biol, P-4150 Porto, Portugal Univ Porto Porto Portugal P-4150 Mol & Cell Biol, P-4150 Porto, Portugal Wageningen Univ, Wageningen Inst Anim Sci, Cell Biol & Immunol Grp, NL-6700 AH Wageningen, Netherlands Wageningen Univ Wageningen Netherlands NL-6700 AH ageningen, Netherlands Marine Lab Aberdeen, Aberdeen AB11 9DB, Scotland Marine Lab Aberdeen Aberdeen Scotland AB11 9DB erdeen AB11 9DB, Scotland
Titolo Testata:
FISH & SHELLFISH IMMUNOLOGY
fascicolo: 4, volume: 11, anno: 2001,
pagine: 317 - 331
SICI:
1050-4648(200105)11:4<317:KOJSB(>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROUT ONCORHYNCHUS-MYKISS; SALMO-SALAR L; CARP CYPRINUS-CARPIO; RAINBOW-TROUT; ELISPOT-ASSAY; AEROMONAS-SALMONICIDA; IMMUNE-RESPONSE; INDUCED SUPPRESSION; YERSINIA-RUCKERI; FISH;
Keywords:
fish; sea bass; Dicentrarchus labrax; ELISPOT; antibody-secreting cells; kinetics; Photobacterium damselae ssp piscicida; Vibrio anguillarum; DNP-KLH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Dos Santos, NMS Univ Porto, Inst Mol & Cell Biol, Rua Campo Alegre 823, P-4150 Porto, Portugal Univ Porto Rua Campo Alegre 823 Porto Portugal P-4150gal
Citazione:
N.M.S. Dos Santos et al., "Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP)", FISH SHELLF, 11(4), 2001, pp. 317-331

Abstract

The ELISPOT assay was used to measure the number of specific antibody secreting cells (ASC) induced during the primary and secondary immune responsesin the spleen, head kidney and gut of juvenile (5 g) sea bass (Dicentrarchus labrax) to bacterial (Vibrio anguillarum and Photobacterium damselae ssp. piscicida) and hapten dinitrophenyl-conjugated to keyhole limpet haemocyanin (DNP-KLH) antigens administered intraperitoneally. High variability among individuals was observed at each sampling day. All fish were bath vaccinated to V. anguillarum at an earlier stage (2 g) in the farm of origin prior to the development of the experiments, and therefore only secondary and tertiary responses were measured in the group immunised with this bacterium. Significant differences to the controls were observed in the primary responses of the head kidney and the spleen to P. damselae ssp. piscicida and DNP, respectively. Frequency analysis of the production of ASC suggests that significant responses in the gut might be masked by the high error variance. The peak of the primary response was observed 4 days earlier to DNP (18-20 days post-immunisation) and it was significantly higher than the responseto P. damselae ssp. piscicida. Higher numbers of ASC were observed in the secondary responses of the head kidney and spleen, although they were not statistically significantly different from the primary levels, probably due to the high error variance as supported by the frequency analysis. Nevertheless, together with a faster response (peak at 7 days post-immunisation), the data suggest that memory formation had occurred. Additionally, the data suggest that some suppression of the secondary immune response in the gut might have occurred. The head kidney appears to produce the highest number of specific ASC of the organs tested. It appears that sea bass show a relatively fast but short duration antibody response. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:07:03