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Titolo:
Intercellular distribution of aberrations detected by means of chromosome painting in cells of patients with cancer prone chromosome instability syndromes
Autore:
Arutyunyan, R; Neubauer, S; Martus, P; Dork, T; Stumm, M; Gebhart, E;
Indirizzi:
Yerevan State Univ, Dept Genet & Cytol, Yerevan 375049, Armenia Yerevan State Univ Yerevan Armenia 375049 Cytol, Yerevan 375049, Armenia Univ Erlangen Nurnberg, Clin & Polyclin Radiat Therapy, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Univ Erlangen Nurnberg, Inst Med Informat Biometry & Epidemiol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Zentrum Kinderheilkunde & Humangenet, Inst Humangenet, D-30625 Hannover, Germany Zentrum Kinderheilkunde & Humangenet Hannover Germany D-30625 r, Germany Univ Klin Magdeburg, Inst Humangenet, D-39129 Magdeburg, Germany Univ KlinMagdeburg Magdeburg Germany D-39129 D-39129 Magdeburg, Germany Univ Erlangen Nurnberg, Inst Human Genet, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
EXPERIMENTAL ONCOLOGY
fascicolo: 1, volume: 23, anno: 2001,
pagine: 23 - 28
SICI:
0204-3564(200103)23:1<23:IDOADB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIATION-INDUCED TRANSLOCATIONS; IN-SITU HYBRIDIZATION; INSITU HYBRIDIZATION; BLOOD-LYMPHOCYTES; DNA PROBES; PERSISTENCE;
Keywords:
cancer patients; ataxia telangiectasia; Nijmegen breakage syndrome; lymphocyte culture; lympoblastoid cell lines; chromosome painting; chromosomal aberrations; intercellular distribution;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Arutyunyan, R Yerevan State Univ, Dept Genet & Cytol, Yerevan 375049, Armenia Yerevan State Univ Yerevan Armenia 375049 375049, Armenia
Citazione:
R. Arutyunyan et al., "Intercellular distribution of aberrations detected by means of chromosome painting in cells of patients with cancer prone chromosome instability syndromes", EXP ONCOL, 23(1), 2001, pp. 23-28

Abstract

A three-color chromosome in situ suppression (CISS) hybridization technique (chromosome painting) was applied to analyze spontaneous and induced (by in vitro irradiation) chromosomal aberrations in peripheral lymphocytes andlymphoblastoid cell lines of homozygote and heterozygote individuals with ataxia telangiectasia (AT) and Nijmegen breakage syndrome (NBS). The painting technique employing for hybridization DNA libraries of the chromosomes 1, 2, and 4 allows the additional detection of chromosomal rearrangements which are not or at least not easily detectable by the classical cytogenetic methods. In all investigated groups the observed intercellular distribution of chromosomal aberrations and breaks was compared with Poisson distribution and geometric distribution. In the lymphocytes of homozygous patients with AT a very high level of chromosomal aberrations and breaks was induced by the irradiation of cultures at a dose of 2.0 Gy, as compared with AT heterozygotes irradiated with the same dose. The observed distributions of chromosomal aberrations in cells fitted Poisson distribution in the absolute majority of variants, while geometric distribution fitted much lesser number of variants. In AT heterozygotes individuals levels of chromosomal aberrations induced by in vitro irradiation at a dose of 2.0 Gy were lower than in AT homozygotes, and their observed distributions were mainly described by geometricdistribution and only in a few cases by Poisson distribution. In one NBS homozygous patient the comparatively low level of aberrations per cell upon irradiation at a dose of 2.0 Gy was described by geometric distribution, while under identical experimental conditions, in the group of NBS heterozygous individuals the low levels of chromosomal aberrations per cell were described both by Poisson and geometric distribution. The low levels of spontaneous and radiation-induced chromosomal aberrations in all investigated groups fitted both distributions. The distributions of breaks in the absolute majority of irradiated variants fitted neither Poisson nor geometric distribution requiring further comparison with other distributions or combination of distributions. The problems of description of observed distributions by the theoretical ones are discussed taking into account specific experimental conditions or nonspecific parameters, Comparative analysis of intercellular distributions could be regarded as an informative cytogenetic approach to the research of radiosensitivity in the patients with chromosomal instability.

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Documento generato il 26/09/20 alle ore 11:51:26