Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of leptin on secretion of LH and FSN from primary cultured female rat pituitary cells
Autore:
Ogura, K; Irahara, M; Kiyokawa, M; Tezuka, M; Matsuzaki, T; Yasui, T; Kamada, M; Aono, T;
Indirizzi:
Univ Tokushima, Sch Med, Dept Obstet & Gynecol, Tokushima 7708503, Japan Univ Tokushima Tokushima Japan 7708503 Gynecol, Tokushima 7708503, Japan
Titolo Testata:
EUROPEAN JOURNAL OF ENDOCRINOLOGY
fascicolo: 6, volume: 144, anno: 2001,
pagine: 653 - 658
SICI:
0804-4643(200106)144:6<653:EOLOSO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
OBESE GENE-PRODUCT; BODY-MASS INDEX; PUBERTAL DEVELOPMENT; SEXUAL-MATURATION; PLASMA LEPTIN; FAT MASS; RECEPTOR; ONSET; MICE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ogura, K Univ Tokushima, Sch Med, Dept Obstet & Gynecol, 3-18-15 Kuramoto Cho, Tokushima 7708503, Japan Univ Tokushima 3-18-15 Kuramoto Cho Tokushima Japan 7708503 apan
Citazione:
K. Ogura et al., "Effects of leptin on secretion of LH and FSN from primary cultured female rat pituitary cells", EUR J ENDOC, 144(6), 2001, pp. 653-658

Abstract

Background: Leptin, which is the product of the obese gene, is believed toplay important roles in pubertal development and reproductive function in females. In a study using adult male rats, it was found that leptin stimulated secretion of gonadotropin from the pituitary in a dose-related manner. However, there has been no such study in female rats. Objective: To investigate the effects of leptin on the production of LH and FSH from the pituitary in female rats, using primary cultured pituitary cells. Methods: In this study, we determined body weight. serum leptin concentration and serum estradiol (E-2) concentration in female Wistar rats at 3, 5, 6, 7, 9 and 11 weeks of age, and cultured pituitary cells from 6-week-old female Wistar rats with leptin (0-10(-7) mol/l) and GnRH (0 or 10(-8) mol/l). Then basal and GnRH-stimulated extra- and intracellular LH and FSH were assayed by RIA. Results: Serum leptin concentration increased with increases in body weight and E-2 concentration. The pubertal serum leptin concentration was about 10(-10) mol/l. At a lower or moderate concentration, leptin produced dose-related increases in both basal and GnRH-stimulated extra- and intracellularLH and FSH in pituitary cells. At a concentration of 10(-10) mol/l, leptinsignificantly (P < 0.05) stimulated both basal and GnRH-stimulated extra- and intracellular LH and FSH. However, at greater concentrations, these effects diminished. Conclusions: These results indicated that leptin induced pituitary cells to produce and secrete both LH and FSH, with or without GnRH. The concentration of leptin that induced the greatest production of gonadotropins by pituitary cells was 10(-10) mol/l, which was the same as the physiological pubertal concentration. Leptin may be involved in the onset of puberty. It is also conceivable that leptin may be a cause of ovulatory failure, not only in weight loss but also in weight gain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:19:07