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Titolo:
Heat shock protein 70 levels in rainbow trout primary epidermal cultures in response to 2,4-dichloroaniline exposure: A novel In vitro aquatic toxicity marker
Autore:
Kilemade, M; Mothersill, C;
Indirizzi:
Dublin Inst Technol, Environm & Radiat Sci Ctr, Dublin 8, Ireland Dublin Inst Technol Dublin Ireland 8 & Radiat Sci Ctr, Dublin 8, Ireland
Titolo Testata:
ENVIRONMENTAL TOXICOLOGY
fascicolo: 3, volume: 16, anno: 2001,
pagine: 253 - 259
SICI:
1520-4081(200106)16:3<253:HSP7LI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ONCORHYNCHUS-MYKISS; EPITHELIAL-CELLS; CADMIUM EXPOSURE; BROWN TROUT; FISH; STRESS; TRANSPORT; EXPLANTS; GROWTH; SKIN;
Keywords:
primary epidermal trout cultures; HSP 70; sublethal risk measurement; immunocytochemistry; biomarker;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Kilemade, M Dublin Inst Technol, Environm & Radiat Sci Ctr, Kevin St, Dublin 8, Ireland Dublin Inst Technol Kevin St Dublin Ireland 8 blin 8, Ireland
Citazione:
M. Kilemade e C. Mothersill, "Heat shock protein 70 levels in rainbow trout primary epidermal cultures in response to 2,4-dichloroaniline exposure: A novel In vitro aquatic toxicity marker", ENVIRON TOX, 16(3), 2001, pp. 253-259

Abstract

The aim of this work was to investigate the use of the heat shock protein,HSP 70, as a sublethal measurement of ecotoxicity and to identify if the amount of HSP 70 synthesized is proportional to the chemical stress applied. This was achieved by quantifying the HSP 70 levels in primary cultured rainbow trout, Oncorhynchus mykiss (R.), skin epidermal cells in response to 2,4-dichloroaniline (2,4-DCA) exposure. The cellular stress response protects organisms from damage resulting from exposure to a wide variety of stressors including xenobiotics. The use of a HSP 70 polyclonal antibody on rainbow trout primary epidermal skin cultures exposed to 2,4-DCA was investigated as a possible biomarker for environmental stress using an immunocytochemical approach. The epidermis is highly susceptible, as it is the interface between the fish and its aquatic environment. In this study we have developed a simple in vitro system for aquatic-toxicity risk assessment. A method for the quantification of heat shock (stress) protein levels by immunocytochemistry is described. The antibody dilution range enabled the detection andquantification of only the inducible HSP 70 fraction. A 1:2000 dilution was decided upon. This assay was effective in detecting and quantifying the induced HSP 70. There was a direct toxicant concentration-dependent increasein the levels of the cellular stress protein in the primary epidermal cultures. Enhanced localization of HSP 70 in the nuclei of the epidermal cells was observed following exposure to 2,4-DCA. This work indicated the possibility of using heat shock protein induction and subsequent quantification asa sensitive system for aquatic toxicity risk assessment. (C) 2001 by John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 23:52:48