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Titolo:
Identification of a P2X7 receptor in GH(4)C(1) rat pituitary cells: A potential target for a bioactive substance produced by Pfiesteria piscicida
Autore:
Kimm-Brinson, KL; Moeller, PDR; Barbier, M; Glasgow, H; Burkholder, JM; Ramsdell, JS;
Indirizzi:
NOAA, Coastal Res Branch,Ctr Coastal Environm Hlth & Bi, Natl Ocean Serv, Marine Biotoxins Program, Charleston, SC 29412 USA NOAA Charleston SC USA 29412 Biotoxins Program, Charleston, SC 29412 USA N Carolina State Univ, Dept Bot, Raleigh, NC 27695 USA N Carolina State Univ Raleigh NC USA 27695 ept Bot, Raleigh, NC 27695 USA
Titolo Testata:
ENVIRONMENTAL HEALTH PERSPECTIVES
fascicolo: 5, volume: 109, anno: 2001,
pagine: 457 - 462
SICI:
0091-6765(200105)109:5<457:IOAPRI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR ATP; MICROGLIAL CELLS; P2X(7) RECEPTOR; PLASMA-MEMBRANE; ESTUARINE FISH; P2Z RECEPTOR; DINOFLAGELLATE; MACROPHAGES; EXPRESSION; EXPOSURE;
Keywords:
c-fos; GH(4)C(1); P2X7; Pfiesteria; pituitary; purinergic; toxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ramsdell, JS NOAA, Coastal Res Branch,Ctr Coastal Environm Hlth & Bi, NatlOcean Serv, Marine Biotoxins Program, 219 Ft Johnson Rd, Charleston, SC 29412 USA NOAA 219 Ft Johnson Rd Charleston SC USA 29412 , SC 29412 USA
Citazione:
K.L. Kimm-Brinson et al., "Identification of a P2X7 receptor in GH(4)C(1) rat pituitary cells: A potential target for a bioactive substance produced by Pfiesteria piscicida", ENVIR H PER, 109(5), 2001, pp. 457-462

Abstract

We examined the pharmacologic activity of a putative toxin (pPfTx) produced by Pfesteria piscicida by characterizing the signaling pathways that induce the c-fos luciferase construct in GH(4)C(1) rat pituitary cells. Adenosine-5 ' -triphosphate (ATP) was determined to increase and, at higher concentrations, decrease luciferase activity in GH(4)C(1) rat pituitary cells that stably express c-fos luciferase. The inhibition of luciferase results from cytotoxicity, characteristic of the putative P. piscicida toxin (pPfTx). The actions of both pPfTx and ATP to induce c-fos luciferase were inhibitedby the purinogenic receptor antagonist pyridoxalphosphate-6-azophenyl-2 ' ,4 ' -disulfonic acid (PPADS). Further characterization of a P2X receptor on the GH(4)C(1) cell was determined by the analog selectivity of P2X agonists. The P2X1/P2X3 agonist alpha,beta -methylene ATP (alpha,beta -MeATP) failed to increase or decrease c-fos luciferase. However, the P2X7 agonist 2 ',3 '-(4-benzoyl)benzoyl ATP (BzATP), which had a predominant cytotoxic effect, was more potent than ATP. Immunoblot analysis of GH(4)C(1) cell membranes confirmed the presence of a 70-kDa protein that was immunoreactive to an antibody directed against the carboxy-terminal domain unique to the P2X7 receptor. The P2X7 irreversible antagonist oxidized-ATP (oxATP) inhibited the action of ATP, BzATP, and pPfTx. These findings indicate that GH(4)C(1) cells express purinogenic receptors with selectivity consistent with the P2X7 subtype and that this receptor pathway mediates the induction of the c-fos luciferase reporter gene by ATP and the putative Pfiesteria toxin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:42:55